Application of flat panel OLED display technology for the point-of-care detection of circulating cancer biomarkers.
Application of flat panel OLED display technology for the point-of-care detection of circulating cancer biomarkers.
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DOI：
10.1038/srep29057
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发表时间：
2016-07-04
影响因子：
4.6
通讯作者：
Anderson KS
中科院分区：
文献类型：
--
作者：
Katchman BA;Smith JT;Obahiagbon U;Kesiraju S;Lee YK;O'Brien B;Kaftanoglu K;Blain Christen J;Anderson KS
床旁分子诊断可以提供高效和具有成本效益的医疗护理，它们有可能从根本上改变我们对全球健康的态度。然而，大多数现有的方法不能扩展到包括多种生物标志物。作为一种解决方案，我们将商用平板OLED显示技术与蛋白质微阵列技术相结合，以紧凑的一次性配置实现高密度荧光、可编程、多路复用的生物识别，并具有临床水平的灵敏度。我们的方法利用商业显示技术的进步，将预功能化的生物传感器基板成本降低到每平方厘米几美分。在这里，我们证明了对患者血清样本中多种病毒抗原的IgG抗体的定量检测，人IgG的检测限在10 pg/mL范围内。我们还证明了对HPV 16蛋白E2、E6和E7抗体的多重检测，这些蛋白是宫颈癌以及头颈癌的循环生物标志物。
Point-of-care molecular diagnostics can provide efficient and cost-effective medical care, and they have the potential to fundamentally change our approach to global health. However, most existing approaches are not scalable to include multiple biomarkers. As a solution, we have combined commercial flat panel OLED display technology with protein microarray technology to enable high-density fluorescent, programmable, multiplexed biorecognition in a compact and disposable configuration with clinical-level sensitivity. Our approach leverages advances in commercial display technology to reduce pre-functionalized biosensor substrate costs to pennies per cm2. Here, we demonstrate quantitative detection of IgG antibodies to multiple viral antigens in patient serum samples with detection limits for human IgG in the 10 pg/mL range. We also demonstrate multiplexed detection of antibodies to the HPV16 proteins E2, E6, and E7, which are circulating biomarkers for cervical as well as head and neck cancers.