INDUCTION OF METAPHASE ARREST IN CLEAVING XENOPUS-EMBRYOS BY MAP KINASE
INDUCTION OF METAPHASE ARREST IN CLEAVING XENOPUS-EMBRYOS BY MAP KINASE
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DOI：
10.1126/science.8235656
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发表时间：
1993-11-19
期刊：
影响因子：
56.9
通讯作者：
MALLER, JL
中科院分区：
文献类型：
--
作者：
HACCARD, O;SARCEVIC, B;MALLER, JL
脊椎动物未受精卵在第二次减数分裂中期的自然停滞是细胞生长抑制因子（CSF）活性的结果。 c-mos(xe) 原癌基因的产物被认为是脑脊液的组成部分，当注射到两细胞胚胎的卵裂球中时，可以诱导中期停滞。 c-Mos(xe)蛋白在体外可以直接激活丝裂原激活蛋白激酶激酶（MAP激酶激酶），从而导致MAP激酶的激活。 MAP 激酶和 c-Mos(xe) 在未受精卵中活跃，受精后迅速失活。将硫代磷酸化 MAP 激酶显微注射到两细胞胚胎的一个卵裂球中，可诱导与 c-Mos(xe) 诱导的中期停滞相似的中期停滞。然而，只有 c-Mos(xe) 的抑制与内源 MAP 激酶的激活相关。这些结果表明活性 MAP 激酶是非洲爪蟾 CSF 的组成部分，并表明 C-Mos(xe) 的 CSF 活性是由 MAP 激酶介导的。
The natural arrest of vertebrate unfertilized eggs in second meiotic metaphase results from the activity of cytostatic factor (CSF). The product of the c-mos(xe) proto-oncogene is thought to be a component of CSF and can induce metaphase arrest when injected into blastomeres of two-cell embryos. The c-Mos(xe) protein can directly activate the mitogen-activated protein kinase kinase (MAP kinase kinase) in vitro, leading to activation of MAP kinase. MAP kinase and c-Mos(xe) are active in unfertilized eggs and are rapidly inactivated after fertilization. Microinjection of thiophosphorylated MAP kinase into one blastomere of a two-cell embryo induced metaphase arrest similar to that induced by c-Mos(xe). However, only arrest with c-Mos(xe) was associated with activation of endogenous MAP kinase. These results indicate that active MAP kinase is a component of CSF in Xenopus and suggest that the CSF activity of C-Mos(xe) is mediated by MAP kinase.