Microcalorimetry of biological macromolecules
Microcalorimetry of biological macromolecules
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DOI：
10.1016/j.bpc.2006.05.004
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发表时间：
2007-03-01
影响因子：
3.8
通讯作者：
Dragan, Anatoly I.
中科院分区：
文献类型：
--
作者：
Privalov, Peter L.;Dragan, Anatoly I.
讨论了当代差示扫描和等温滴定微量热法研究蛋白质去折叠/重折叠的热力学及其与伴侣，特别是靶DNA双链的相互作用。结果表明，在研究蛋白质天然结构及其与伴侣形成的络合物，特别是它们的同源DNA双链形成的热力学时，蛋白质的变性前的变化是不容忽视的。(C)2006爱思唯尔B.V.保留所有权利。
The capabilities of contemporary differential scanning and isothermal titration microcalorimetry for studying the thermodynamics of protein unfolding/refolding and their association with partners, particularly target DNA duplexes, are considered. It is shown that the predenaturational changes of proteins must not be ignored in studying the thermodynamics of formation of their native structure and their complexes with partners, particularly their cognate DNA duplexes. (c) 2006 Elsevier B.V. All rights reserved.