Myelin-targeted, texaphyrin-based multimodal imaging agent for magnetic resonance and optical imaging.
Myelin-targeted, texaphyrin-based multimodal imaging agent for magnetic resonance and optical imaging.
复制标题
用于磁性共振和光学成像的基于髓磷脂的基于Texaphyrin的多模式成像剂。
DOI：
10.1002/cmmi.1711
复制
发表时间：
2016-11
影响因子：
--
通讯作者：
Allen MJ
中科院分区：
文献类型：
--
作者：
Vithanarachchi SM;Foley CD;Trimpin S;Ewing JR;Ali MM;Allen MJ
可靠的髓鞘成像方法对于研究脱髓鞘的原因和研究促进髓鞘再生的药物至关重要。髓鞘特异性化合物可以被开发成成像探针，以利用各种成像技术检测髓鞘。多模式髓鞘特异性成像探针的开发使得能够使用正交成像技术来准确地可视化髓鞘含量并验证实验结果。在这里，我们描述了多模式髓鞘特异性成像剂的光学显微镜和磁共振成像的合成和应用。成像剂的合成，将结构特征的luxol坚牢蓝MBS，髓鞘特异性组织染色，到texaphyrins协调Gd III。这些新的复合物表现出可见光的吸收，近红外光的发射，和弛豫值大于临床批准的磁共振成像造影剂。这些特性使得能够使用光学成像和磁共振成像来可视化髓鞘。我们对离体小鼠脑进行切片和封闭染色，以研究新化合物对髓鞘的特异性。从光学显微镜和磁共振成像获得的图像表明，我们的复合物保留在白色物质结构，并使髓鞘检测。
Reliable methods of imaging myelin are essential to investigate the causes of demyelination and to study drugs that promote remyelination. Myelin-specific compounds can be developed into imaging probes to detect myelin with various imaging techniques. The development of multimodal myelin-specific imaging probes enables the use of orthogonal imaging techniques to accurately visualize myelin content and validate experimental results. Here, we describe the synthesis and application of multimodal myelin-specific imaging agents for light microscopy and magnetic resonance imaging. The imaging agents were synthesized by incorporating the structural features of luxol fast blue MBS, a myelin-specific histological stain, into texaphyrins coordinated to GdIII. These new complexes demonstrated absorption of visible light, emission of near-IR light, and relaxivity values greater than clinically approved contrast agents for magnetic resonance imaging. These properties enable the use of optical imaging and magnetic resonance imaging for visualization of myelin. We performed section- and en block-staining of ex vivo mouse brains to investigate the specificity for myelin of the new compounds. Images obtained from light microscopy and magnetic resonance imaging demonstrate that our complexes are retained in white matter structures and enable detection of myelin.