Analysis of biochemical variations and microRNA expression in wild (Ipomoea campanulata) and cultivated (Jacquemontia pentantha) species exposed to in vivo water stress
Analysis of biochemical variations and microRNA expression in wild (Ipomoea campanulata) and cultivated (Jacquemontia pentantha) species exposed to in vivo water stress
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DOI：
10.1007/s12298-013-0207-1
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发表时间：
2014-01-01
影响因子：
3.5
通讯作者：
Krishnayya, N. S. R.
中科院分区：
文献类型：
--
作者：
Ghorecha, Vallabhi;Patel, Ketan;Krishnayya, N. S. R.
目前的研究分析了两个密切相关的物种(野生但耐性的钟形通菜和栽培的但敏感的五叶野茉莉)在田间自然缺水条件下的几个重要生化参数和microRNA的表达。在土壤水分亏缺条件下，这两个树种的叶面积和SLA都比水分充足时有所降低。在胁迫下，五角莲的叶绿素含量下降幅度较大(接近50%)，而风铃藻的降幅较大(20%)。相比之下，五花莲的花色苷和丙二醛的积累量要比钟形花序的大。这两种植物在胁迫下观察到多种不同活性的超氧化物歧化酶(SOD)同工酶。铜锌超氧化物歧化酶亚型在风铃菊中的诱导率相对较高(接近10-40%)。MicroRNAs、miR398、miR319、miR395、miR172和miR408在水分亏缺条件下表现出相反的表达。MiR156、MIR168、miR171、miR172、miR393、miR319、miR396、miR397和miR408的表达与拟南芥的干旱胁迫表现出相反的表达模式。野生种(I.Campanulata)对水分亏缺的耐受性较好，这可能归因于叶绿素和花青素水平的变化较小，以及相对较高的SOD水平。两种植物的miRNA表达差异较大。
The current study analyses few important biochemical parameters and microRNA expression in two closely related species (wild but tolerant Ipomoea campanulata L. and cultivated but sensitive Jacquemontia pentantha Jacq.G.Don) exposed to water deficit conditions naturally occurring in the field. Under soil water deficit, both the species showed reduction in their leaf area and SLA as compared to well-watered condition. A greater decrease in chlorophyll was noticed in J. pentantha (similar to 50 %) as compared to I. campanulata (20 %) under stress. By contrast, anthocyanin and MDA accumulation was greater in J. pentantha as compared to I. campanulata. Multiple isoforms of superoxide dismutases (SODs) with differing activities were observed under stress in these two plant species. CuZnSOD isoforms showed comparatively higher induction (similar to 10-40 %) in I. campanulata than J. pentantha. MicroRNAs, miR398, miR319, miR395 miR172, and miR408 showed opposing expression under water deficit in these two plant species. Expression of miR156, miR168, miR171, miR172, miR393, miR319, miR396, miR397 and miR408 from either I. campanulata or J. pentantha or both demonstrated opposite pattern of expression to that of drought stressed Arabidopsis. The better tolerance of the wild species (I. campanulata) to water deficit could be attributed to lesser variations in chlorophyll and anthocyanin levels; and relatively higher levels of SODs than J. pentantha. miRNA expression was different in I. campanulata than J. pentantha.