Synthetic peptides of the Rab effector domain inhibit vesicular transport through the secretory pathway.
Synthetic peptides of the Rab effector domain inhibit vesicular transport through the secretory pathway.
复制标题
Rab 效应结构域的合成肽通过分泌途径抑制囊泡运输。
作者：
H. Plutner;R. Schwaninger;S. Pind;W. E. Balch
合成了ras相关的Rab基因家族小GTP结合蛋白的效应域的合成肽，在体外发现它是内质网(ER)对高尔基体和高尔基体内转运的有效抑制物。其中一个效应域肽对转运的抑制作用迅速(30个S中的1/2)，且不可逆。对多肽抑制的时间位置的分析表明，转运的较晚步骤被阻止，这与依赖钙离子的灌流步骤一致。这些结果为Rab基因家族成员在哺乳动物细胞囊泡运输中的作用提供了新的生化证据，并暗示了新的下游Rab效应蛋白(REP)调节囊泡融合的作用。
Synthetic peptides of the putative effector domain of members of the ras‐related rab gene family of small GTP‐binding proteins were synthesized and found to be potent inhibitors of endoplasmic reticulum (ER) to Golgi and intra‐Golgi transport in vitro. Inhibition of transport by one of the effector domain peptides was rapid (t1/2 of 30 s), and irreversible. Analysis of the temporal site of peptide inhibition indicated that a late step in transport was blocked, coincident with a Ca2(+)‐dependent prefusion step. The results provide novel biochemical evidence for the role of members of the rab gene family in vesicular transport in mammalian cells, and implicate a role for a new downstream Rab effector protein (REP) regulating vesicle fusion.