Identification of genes involved in color variation of bamboo culms by suppression subtractive hybridization.
Identification of genes involved in color variation of bamboo culms by suppression subtractive hybridization.
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DOI：
10.1016/j.plaphy.2015.10.004
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发表时间：
2015-12
期刊：
影响因子：
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通讯作者：
X. Xia;R. Gui;Haiyun Yang;Ying Fu;Fang Wei;Mingbing Zhou
中科院分区：
文献类型：
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作者：
X. Xia;R. Gui;Haiyun Yang;Ying Fu;Fang Wei;Mingbing Zhou
间日竹aureocaulis 是一种广泛种植的观赏竹，叶子常绿。这种植物的茎呈现金黄色背景颜色，随机标记有窄和宽的绿色条纹，但偶尔也有浅绿色和黄色条纹。在这项研究中，我们试图确定条纹秆颜色变化背后的分子机制。我们发现在黄色组织细胞的质体中既没有观察到基质也没有观察到基粒片层，而在绿色组织中观察到完整的叶绿体。此外，叶绿素a和b主要分布在表皮下的地组织和节间绿色部分的束鞘周围的细胞中。秆绿色部分横截面所含的叶绿素含量显着高于黄色部分。然而，两个样品中几乎检测不到类胡萝卜素。此外，我们发现PvESTs-F641（JZ893845）、PvESTs-F681（JZ893885）和PvESTs-F798（JZ894002）等7个ESTs在绿色样本中的表达水平显着高于黄色样本，而PvESTs-R200（JZ894906）、在黄色样品中发现 PvESTs-R541 (JZ895247)、PvESTs-R333 (JZ895039) 和 PvESTs-R266 (JZ894972) 含量较高。这些 EST 被认为在植物颜色变化中发挥了作用。我们目前的结果表明，黄色组织中光合膜蛋白和脂质不足可能导致叶绿体功能障碍，并可能导致某些P出现黄色外观。 间日疟原虫梗茎。
Phyllostachysvivaxcv. aureocaulis is a widely planted ornamental bamboo with evergreen leaves. This plant's culm exhibits a golden-yellow background color marked randomly with narrow and broad green stripes but is occasionally light green with yellow stripes. In this study, we attempt to identify the molecular mechanism underlying the color variation in striped culms. We found that neither stroma nor grana lamellas were observed in plastids in yellow tissue cells, while complete chloroplasts were observed in green tissue. In addition, chlorophyll a and b were mainly distributed in ground tissue under the epiderm and in the cells surrounding the bundle sheath in the green portion of internodes. The amount of chlorophyll contained in cross-sections of the green portion of culms is significantly higher than in the yellow portion. However, carotenoid was nearly undetectable in both samples. In addition, we found that the expression levels of 7 ESTs, including PvESTs-F641 (JZ893845), PvESTs-F681 (JZ893885) and PvESTs-F798 (JZ894002), were significantly higher in green samples than that in yellow samples, while PvESTs-R200 (JZ894906), PvESTs-R541 (JZ895247), PvESTs-R333 (JZ895039) and PvESTs-R266 (JZ894972) were found at a higher level in yellow samples. These ESTs are thought to play a role in this color variation in plants. Our current results indicate that insufficient photosynthetic membrane proteins and lipids in yellow tissue could lead to chloroplast dysfunction and could result in the yellow appearance on certainP. vivax cv. aureocaulis culms.