Drosophila simulans: A Species with Improved Resolution in Evolve and Resequence Studies.
Drosophila simulans: A Species with Improved Resolution in Evolve and Resequence Studies.
复制标题
DOI：
10.1534/g3.117.043349
复制
发表时间：
2017-07-05
期刊：
影响因子：
--
通讯作者：
Schlötterer C
中科院分区：
文献类型：
--
作者：
Barghi N;Tobler R;Nolte V;Schlötterer C
实验进化与汇集个体的高通量测序相结合。进化和重排序（E&R）是一种在受控、复制条件下研究长期遗传变异的适应性的有力方法。然而，对黑腹果蝇的E&R研究经常导致候选snp数量过多，特别是对于复杂性状。在这里，我们对比了D. melanogaster和D. simulans在新的高温环境中适应约60代后的基因组特征。与黑腹海蛞蝓相比，模拟海蛞蝓携带假定选择基因座的区域要明显得多，因此假阳性较少。我们认为，没有分离倒置和较高重组率的物种，如拟马羚，更适合用于旨在表征适应反应的遗传变异的E&R研究。
The combination of experimental evolution with high-throughput sequencing of pooled individuals—i.e., evolve and resequence (E&R)—is a powerful approach to study adaptation from standing genetic variation under controlled, replicated conditions. Nevertheless, E&R studies in Drosophila melanogaster have frequently resulted in inordinate numbers of candidate SNPs, particularly for complex traits. Here, we contrast the genomic signature of adaptation following ∼60 generations in a novel hot environment for D. melanogaster and D. simulans. For D. simulans, the regions carrying putatively selected loci were far more distinct, and thus harbored fewer false positives, than those in D. melanogaster. We propose that species without segregating inversions and higher recombination rates, such as D. simulans, are better suited for E&R studies that aim to characterize the genetic variants underlying the adaptive response.