CPEB-mediated cytoplasmic polyadenylation and the regulation of experience-dependent translation of α-CaMKII mRNA at synapses
CPEB-mediated cytoplasmic polyadenylation and the regulation of experience-dependent translation of α-CaMKII mRNA at synapses
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DOI：
10.1016/s0896-6273(00)80630-3
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发表时间：
1998-11-01
期刊：
影响因子：
16.2
通讯作者：
Richter, JD
中科院分区：
文献类型：
--
作者：
Wu, L;Wells, D;Richter, JD
突触功效的长期变化可能需要树突状mRNA的调节翻译。虽然这种调节的基础是未知的，但似乎有可能在神经元中重现发育中的翻译控制的某些特征。多聚腺苷酸化诱导的卵母细胞mRNA翻译需要顺式作用CPE序列和CPE结合蛋白CPEB。CPEB还存在于海马的树突层、培养的神经元的突触处和成人脑的突触后密度中。α-CaMKII mRNA定位于树突中，是突触可塑性和LTP所必需的，含有两个CPE。这些CPE结合CPEB和介导的多聚腺苷酸化诱导的翻译注射非洲爪蟾卵母细胞。在完整的大脑中，视觉体验诱导α-CaMKII mRNA多聚腺苷酸化和翻译，这表明这一过程可能发生在突触。
Long-term changes in synaptic efficacy may require the regulated translation of dendritic mRNAs. While the basis of such regulation is unknown, it seemed possible that some features of translational control in development could be recapitulated in neurons. Polyadenylation-induced translation of oocyte mRNAs requires the cis-acting CPE sequence and the CPE-binding protein CPEB. CPEB is also present in the dendritic layers of the hippocampus, at synapses in cultured neurons, and in postsynaptic densities of adult brain. alpha-CaMKII mRNA, which is localized in dendrites and is necessary for synaptic plasticity and LTP, contains two CPEs. These CPEs are bound by CPEB and mediate polyadenylation-induced translation in injected Xenopus oocytes. In the intact brain, visual experience induces alpha-CaMKII mRNA polyadenylation and translation, suggesting that this process likely occurs at synapses.