Gentian Kobu-sho-associated virus: a tentative, novel double-stranded RNA virus that is relevant to gentian Kobu-sho syndrome
Gentian Kobu-sho-associated virus: a tentative, novel double-stranded RNA virus that is relevant to gentian Kobu-sho syndrome
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DOI：
10.1007/s10327-012-0423-5
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发表时间：
2013-01-01
影响因子：
1.2
通讯作者：
Sekine, Ken-Taro
中科院分区：
文献类型：
--
作者：
Kobayashi, Kappei;Atsumi, Go;Sekine, Ken-Taro
在龙胆属植物中检测到约23 kbp的病毒样dsRNA，该病毒样dsRNA表现为Kobu-sho综合征，包括茎、节和根上的发育迟缓、节间缩短和肿瘤。核苷酸序列分析表明，该dsRNA与瘟病毒属物种有关，但与任何其他植物病毒无关。它被保护免受外部添加的RNA酶的影响，这表明dsRNA是双糖苷化的。在戊二醛固定的组织的粗匀浆中与先前与龙胆中的Kobu-sho综合征相关的病毒样颗粒共提取dsRNA（Usugi等，Jpn J Phytopathol 76：21-24，2010）。在超过99%的Kobu-sho龙胆个体中检测到RNA序列，但在来自受Kobu-sho影响的田地的明显健康龙胆个体中检测到的RNA序列不到20%。因此，我们建议将该病毒命名为GARMANKobu-sho相关病毒。
A virus-like dsRNA of about 23 kbp was detected in gentian plants showing Kobu-sho syndrome including stunting, shortened internodes, and tumors on stems, nodes and roots. Nucleotide sequence analysis has suggested that this dsRNA is related to Pestivirus species but not to any other plant viruses. It was protected from externally added RNase, suggesting that the dsRNA is encapsidated. The dsRNA was co-extracted in a crude homogenate of glutaraldehyde-fixed tissue with the virus-like particles that have been associated previously with Kobu-sho syndrome in gentian (Usugi et al. Jpn J Phytopathol 76:21-24, 2010). The RNA sequence was detected in more than 99 % of Kobu-sho gentian individuals but in less than 20 % of apparently healthy gentian individuals from fields affected with Kobu-sho. Thus, we propose naming the virus Gentian Kobu-sho-associated virus.