Conserved ser residues, the shutter region, and speciation in serpin evolution
Conserved ser residues, the shutter region, and speciation in serpin evolution
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DOI：
10.1074/jbc.m305088200
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发表时间：
2003-09-26
影响因子：
4.8
通讯作者：
Di Cera, E
中科院分区：
文献类型：
--
作者：
Krem, MM;Di Cera, E
丝氨酸蛋白酶抑制剂超家族的丝氨酸蛋白酶抑制剂的自杀抑制机制在很大程度上取决于它们的结构灵活性，这在很大程度上是由中央Abeta-折叠的缺口和快门区域控制的。我们检查了密码子使用的高度保守的残基，Ser-53和Ser-56，快门区域，并发现一个TCN-AGY使用二分法的Ser-56，显着地连接到原口-后口分裂。我们的研究结果表明，丝氨酸蛋白酶抑制剂的进化是由系统发育的物种形成，而不是压力，以履行新的生理功能，减轻对共同进化的丝氨酸蛋白酶家族，他们抑制。
The suicide inhibitory mechanism of serine protease inhibitors of the serpin superfamily depends heavily on their structural flexibility, which is controlled in large part by the breach and shutter regions of the central Abeta-sheet. We examined codon usage by the highly conserved residues, Ser-53 and Ser-56, of the shutter region and found a TCN-AGY usage dichotomy for Ser-56 that remarkably is linked to the protostome-deuterostome split. Our results suggest that serpin evolution was driven by phylogenetic speciation and not pressure to fulfill new physiologic functions mitigating against co-evolution with the family of serine proteases they inhibit.