Sequence-specific methyltransferase-induced labelling (SMILing) of plasmid DNA for studying cell transfection
Sequence-specific methyltransferase-induced labelling (SMILing) of plasmid DNA for studying cell transfection
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DOI：
10.1016/j.bmc.2007.04.054
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发表时间：
2008-01-01
影响因子：
3.5
通讯作者：
Weinhold, Elmar
中科院分区：
文献类型：
--
作者：
Schmidt, Falk H. -G.;Hueben, Michael;Weinhold, Elmar
使用新合成的N-腺苷氮丙啶辅因子和DNA甲基转移酶M. TaqI，用Cy 3荧光团对质粒DNA（pUC 19和pBR 322）进行序列特异性共价标记。将荧光标记的质粒用于转染哺乳动物细胞，并通过落射荧光和共聚焦荧光显微镜观察其细胞内分布。虽然这些原核质粒不含核输入序列，但观察到易位到核中。(C)2007爱思唯尔有限公司版权所有。
Plasmid DNA (pUC19 and pBR322) was sequence-specifically, covalently labelled with Cy3 fluorophores using a newly synthesised N-adenosylaziridine cofactor and the DNA methyltransferase M.TaqI. The fluorescently labelled plasmids were used for transfection of mammalian cells and their intracellular distribution was visualised by epifluorescence and confocal fluorescence microscopy. Although these prokaryotic plasmids do not contain nuclear import sequences, translocation into the nuclei was observed. (C) 2007 Elsevier Ltd. All rights reserved.