Adaptive harmonic generation microscopy of mammalian embryos.
Adaptive harmonic generation microscopy of mammalian embryos.
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DOI：
10.1364/ol.34.003154
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发表时间：
2009-10-15
期刊：
影响因子：
3.6
通讯作者：
Booth M
中科院分区：
文献类型：
--
作者：
Jesacher A;Thayil A;Grieve K;Débarre D;Watanabe T;Wilson T;Srinivas S;Booth M
自适应光学实现谐波发生显微镜使用波前传感器校正方案。二次和三次谐波强度信号都用作优化度量。通过使用基于Zernike多项式模式的高效优化例程来执行像差校正以补偿系统和微透镜引起的像差。活小鼠胚胎的图像显示出改善的信号水平和分辨率。
Adaptive optics is implemented in a harmonic generation microscope using a wavefront sensorless correction scheme. Both the second- and third-harmonic intensity signals are used as the optimization metric. Aberration correction is performed to compensate both system- and specimen-induced aberrations by using an efficient optimization routine based upon Zernike polynomial modes. Images of live mouse embryos show an improved signal level and resolution.