Vernalization of Oriental hybrid lily 'Sorbonne': changes in physiology metabolic activity and molecular mechanism
Vernalization of Oriental hybrid lily 'Sorbonne': changes in physiology metabolic activity and molecular mechanism
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东方杂交百合“索邦”的春化：生理代谢活性和分子机制的变化
DOI：
10.1007/s11033-014-3545-3
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发表时间：
2014-10-01
影响因子：
2.8
通讯作者：
Lu, Yingmin
中科院分区：
文献类型：
--
作者：
Liu, Xiaohua;Wang, Qing;Lu, Yingmin
除了生理代谢活动观察之外，还使用东方杂交百合“Sorbonne”研究在 4 A 摄氏度下储存期间的分子变化，以解除休眠。从生理机制来看，百合球茎内源脱落酸（ABA）浓度水平随着冷藏时间的延长而降低，保存35 d后保持在稳定水平。可溶性糖浓度水平在冷藏期间也发生变化，随着冷藏时间增加到49 d，可溶性糖浓度升高，随后降低。在分子水平上，构建了一个新的转录组，提供百合休眠释放过程中变异的全面序列分析数据。分析了对照和处理之间差异表达的 34,367 个unigenes。选择14个基因，包括8个MADS-box家族基因、4个植物激素相关基因和2个DNA甲基化基因，通过qRT-PCR鉴定其表达水平。我们的研究结果表明，冷藏过程中ABA水平的下降以及植物激素基因的变化与休眠解除相关； MADS-box家族基因VRN2、FLC、FT、SOC1a以及LFY、MIKC和ARF、MYB转录因子参与百合成花诱导，DNA甲基化与百合低温春化相关。根据目前的研究结果，我们预测植物激素途径、能量代谢途径、春化途径和DNA甲基化在春化过程中发挥重要作用；这些数据为未来研究春化诱导百合开花奠定了基础。
Oriental hybrid lily 'Sorbonne' was used to investigate molecular changes during the storage at 4 A degrees C for dormancy-release besides physiology metabolic activity observations. In physiological mechanism, endogenous abscisic acid (ABA) concentration level of lily bulbs decreased as the cold preservation time increased, and it kept at a stable level after being preserved for 35 days. The level of soluble sugars concentrations also changed during the cold preservation time, and it increased as the cold preservation time raised to 49 days then decreased afterward. On molecule level, a new transcriptome providing comprehensive sequence profiling data of variation during dormancy-release in lily was constructed. 34,367 unigenes expressed differentially between the control and the treatment was analyzed. 14 genes including 8 MADS-box family genes, 4 genes related to plant hormone, and 2 DNA methylation genes were selected to identify the levels of their expression by qRT-PCR. Our results show that the decrease of ABA level during cold storage, as well as changes in plant hormone genes was correlated with dormancy-release; MADS-box family genes VRN2, FLC, FT, SOC1 a, as well as LFY, MIKC and ARF, MYB transcription factor were included in lily floral induction and DNA methylation was correlated to lily vernalization under low temperature. According to the results of the present studies, we predicted that plant hormone pathway, energy metabolic pathway, vernalization pathway, and DNA methylation played important roles during vernalization; these data provided the foundation for future studies of vernalization to induce flowering of lily.