Diversity of DNA β, a satellite molecule associated with some monopartite begomoviruses
Diversity of DNA β, a satellite molecule associated with some monopartite begomoviruses
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DOI：
10.1016/s0042-6822(03)00200-9
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发表时间：
2003-07-20
期刊：
影响因子：
3.7
通讯作者：
Markham, PG
中科院分区：
文献类型：
--
作者：
Briddon, RW;Bull, SE;Markham, PG
DNA β分子是与单分双生病毒（双生病毒科）相关的调节DNA β的单链DNA卫星。这样的分子迄今已显示与来自新加坡的胜红蓟黄脉病毒和来自巴基斯坦的棉花叶短木尔坦病毒相关。在这里，26个额外的DNA β分子，与不同的植物物种从不同的地理位置获得，克隆和测序。这些分子被证明是广泛存在于旧世界，那里的单分裂菜豆花叶病毒是已知的发生。序列分析揭示了DNA β分子的高度保守结构，包括一个保守的开放阅读框架、一个富含腺嘌呤的区域和一个高度序列保守的区域[卫星保守区（SCR）]。SCR含有具有环序列TAA/GTATTAC的潜在发夹结构;类似于双生病毒和纳米病毒的复制起点。通过系统发育分析解决了两个主要的DNA β卫星群。一组起源于锦葵科的宿主，第二组来自茄科和菊科中更多样化的植物。在这两个集群中，DNA β分子显示出基于宿主和地理来源的相关性。这些发现有力地支持DNA β分子与其各自的辅助菜豆花叶病毒的共适应。(C)2003 Elsevier Science（美国）。All rights reserved.
DNA beta molecules are symptom-modulating, single-stranded DNA satellites associated with monopartite begomoviruses (family Geminiviridae). Such molecules have thus far been shown to be associated with Ageratum yellow vein virus from Singapore and Cotton leaf curt Multan virus from Pakistan. Here, 26 additional DNA beta molecules, associated with diverse plant species obtained from different geographical locations, were cloned and sequenced. These molecules were shown to be widespread in the Old World, where monopartite begomoviruses are known to occur. Analysis of the sequences revealed a highly conserved organization for DNA beta molecules consisting of a single conserved open reading frame, an adenine-rich region, and a region of high sequence conservation [the satellite conserved region (SCR)]. The SCR contains a potential hairpin structure with the loop sequence TAA/GTATTAC; similar to the origins of replication of geminiviruses and nanoviruses. Two major groups of DNA beta satellites were resolved by phylogenetic analyses. One group originated from hosts within the Malvaceae and the second from a more diverse group of plants within the Solanaceae and Compositae. Within the two clusters, DNA beta molecules showed relatedness based both on host and geographic origin. These findings strongly support coadaptation of DNA beta molecules with their respective helper begomoviruses. (C) 2003 Elsevier Science (USA). All rights reserved.