Early postweaning exercise improves central leptin sensitivity in offspring of rat dams fed high-fat diet during pregnancy and lactation.
Early postweaning exercise improves central leptin sensitivity in offspring of rat dams fed high-fat diet during pregnancy and lactation.
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DOI：
10.1152/ajpregu.00566.2012
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发表时间：
2013-11
期刊：
影响因子：
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通讯作者：
Bo Sun;Nu-Chu Liang;E. Ewald;R. Purcell;G. Boersma;Jian-qun Yan;T. Moran;K. Tamashiro
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文献类型：
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作者：
Bo Sun;Nu-Chu Liang;E. Ewald;R. Purcell;G. Boersma;Jian-qun Yan;T. Moran;K. Tamashiro
母亲的高脂肪(HF)饮食对后代的代谢表型有长期影响。在这里，我们测定了断奶后运动对孕期和哺乳期饲喂HF饲料的大鼠仔鼠的影响。妊娠SD大鼠在整个孕期和哺乳期均以饲料或HF饲料喂养。所有幼崽在出生后21日开始断奶。在4wk时，雄性仔鼠自由使用跑轮(RW)或保持久坐(SED)3wk，之后所有大鼠保持久坐状态，分为4组：Chow-SED组、Chow-RW组、HF-SED组和HF-RW组。雄性HF后代在PND7阶段体重比Chow幼崽增加更多，并在整个实验期间保持这种体重差异。断奶后三周的锻炼对Chow和HF子代的体重增加都没有影响，但减少了HF子代的肥胖。运动3wk结束时，HF-RW大鼠血浆瘦素水平下降，但与运动结束后9wk的HF-SED大鼠相比，差异无统计学意义。在14周龄时，侧脑室注射瘦素抑制了CHOW-SED组、CHOW-RW组和HF-RW组的摄食量，而对HF-SED组的摄食量没有影响。在死亡时，HF-RW大鼠弓状核中瘦素诱导的磷酸化STAT3水平也高于HF-SED大鼠。母体HF饮食和断奶后运动对成年仔鼠下丘脑神经肽及其受体基因表达均有影响。我们的数据表明，在这个模型中，断奶后的运动可以改善中枢瘦素的敏感性和信号传递。
Maternal high-fat (HF) diet has long-term consequences on the metabolic phenotype of the offspring. Here, we determined the effects of postweaning exercise in offspring of rat dams fed HF diet during gestation and lactation. Pregnant Sprague-Dawley rats were maintained on chow or HF diet throughout gestation and lactation. All pups were weaned onto chow diet on postnatal day (PND) 21. At 4 wk of age, male pups were given free access to running wheels (RW) or remained sedentary (SED) for 3 wk, after which all rats remained sedentary, resulting in four groups: CHOW-SED, CHOW-RW, HF-SED, and HF-RW. Male HF offspring gained more body weight by PND7 compared with CHOW pups and maintained this weight difference through the entire experiment. Three weeks of postweaning exercise did not affect body weight gain in either CHOW or HF offspring, but reduced adiposity in HF offspring. Plasma leptin was decreased at the end of the 3-wk running period in HF-RW rats but was not different from HF-SED 9 wk after the exercise period ended. At 14 wk of age, intracerebroventricular injection of leptin suppressed food intake in CHOW-SED, CHOW-RW, and HF-RW, while it did not affect food intake in HF-SED group. At death, HF-RW rats also had higher leptin-induced phospho-STAT3 level in the arcuate nucleus than HF-SED rats. Both maternal HF diet and postweaning exercise had effects on hypothalamic neuropeptide and receptor mRNA expression in adult offspring. Our data suggest that postweaning exercise improves central leptin sensitivity and signaling in this model.