Cofilin activation during Ca2+-triggered secretion from adrenal chromaffin cells
Cofilin activation during Ca2+-triggered secretion from adrenal chromaffin cells
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DOI：
10.1006/bbrc.2001.5435
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发表时间：
2001-08-24
影响因子：
3.1
通讯作者：
Roth, D
中科院分区：
文献类型：
--
作者：
Birkenfeld, J;Kartmann, B;Roth, D
Cofilin是真核细胞中主要的肌动蛋白解聚蛋白之一，参与许多膜调节活动，如细胞生长和运动。在这里，我们研究了cofilin是否在Ca2+调节的牛肾上腺染色质细胞的去甲肾上腺素分泌时被激活。我们发现通过尼古丁触发胞吐会引起去磷酸化，从而激活cofilin。此外，在通透化的染色质细胞中，Ca2+的单独添加足以触发这两个，调节胞外分泌和辅蛋白激活。这与胞吐过程中肌动蛋白重组所需要的cofilin激活是一致的。(C) 2001学术出版社。
Cofilin is one of the major actin depolymerizing proteins in eukaryotic cells and involved in many membrane modulating activities, such as cell growth and motility. Here we examined whether cofilin is activated upon Ca2+ regulated noradrenalin secretion from bovine adrenal chromaffin cells. We found that triggering exocytosis by nicotine causes a dephosphorylation and thereby activation of cofilin. Furthermore, in permeabilized chromaffin cells the addition of Ca2+ alone is sufficient to trigger both, regulated exocytosis and cofilin activation. This is consistent with cofilin activation being required for actin reorganization during exocytosis. (C) 2001 Academic Press.