Co-cultured methanogen improved the metabolism in the hydrogenosome of anaerobic fungus as revealed by gas chromatography-mass spectrometry analysis.
Co-cultured methanogen improved the metabolism in the hydrogenosome of anaerobic fungus as revealed by gas chromatography-mass spectrometry analysis.
复制标题
气相色谱-质谱分析表明，共培养的产甲烷菌改善了厌氧真菌氢化酶体的代谢
DOI：
10.5713/ajas.19.0649
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Zhu W
中科院分区：
文献类型：
--
作者：
Li Y;Sun M;Li Y;Cheng Y;Zhu W
目的研究与产甲烷菌（索氏甲烷短杆菌）共培养的真菌的代谢变化。方法采用气相色谱-质谱联用技术对厌氧真菌（Pecoramyces sp. F1）细胞及培养上清中的代谢产物进行分析。结果在真菌细胞和培养上清中分别检测到104种和102种代谢产物。偏最小二乘判别分析表明，与产甲烷菌共培养时，真菌细胞和上清液中的代谢产物谱发生了明显的变化。统计学上，共培养的产甲烷菌分别显著影响真菌细胞和上清液中的16种和30种代谢产物（p<0.05）。代谢途径分析表明，与产甲烷菌共培养减少了从丙酮酸在胞质溶胶中的乳酸的生产，并增加了厌氧真菌的氢化酶体中的代谢。柠檬酸盐在与产甲烷菌共培养的真菌的细胞质中积累。结论厌氧真菌与产甲烷菌的共培养是研究产氢微生物和利用氢微生物之间相互作用的良好模型。然而，氢酶体的代谢需要进一步研究，以获得更好的了解微生物之间的氢转移。
Objective The purpose of this study was to reveal the metabolic shift in the fungus co-cultured with the methanogen (Methanobrevibacter thaueri). Methods Gas chromatography-mass spectrometry was used to investigate the metabolites in anaerobic fungal (Pecoramyces sp. F1) cells and the supernatant. Results A total of 104 and 102 metabolites were detected in the fungal cells and the supernatant, respectively. The partial least squares-discriminant analysis showed that the metabolite profiles in both the fungal cell and the supernatant were distinctly shifted when co-cultured with methanogen. Statistically, 16 and 30 metabolites were significantly (p<0.05) affected in the fungal cell and the supernatant, respectively by the co-cultured methanogen. Metabolic pathway analysis showed that co-culturing with methanogen reduced the production of lactate from pyruvate in the cytosol and increased metabolism in the hydrogenosomes of the anaerobic fungus. Citrate was accumulated in the cytosol of the fungus co-cultured with the methanogen. Conclusion The co-culture of the anaerobic fungus and the methanogen is a good model for studying the microbial interaction between H2-producing and H2-utilizing microorganisms. However, metabolism in hydrogenosome needs to be further studied to gain better insight in the hydrogen transfer among microorganisms.