Role of the liver in peripheral tolerance: Induction through oral antigen feeding
Role of the liver in peripheral tolerance: Induction through oral antigen feeding
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DOI：
10.1111/j.1600-6143.2004.00551.x
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发表时间：
2004-10-01
影响因子：
8.8
通讯作者：
Perkins, JD
中科院分区：
文献类型：
--
作者：
Li, W;Chou, ST;Perkins, JD
利用小鼠肝移植模型，我们研究了肝脏在外周耐受中的作用。以低剂量或高剂量卵清蛋白（OVA）喂养BALB/c小鼠，将其肝脏移植到同基因受体中。不进食的受体为对照。原位肝移植（OLTx）后进行OVA免疫和迟发型超敏反应（DTH）激发。检测了来自OVA喂养小鼠的肝非实质细胞（NPC）或脾细胞（SC）的离体过继转移效应。对移植受体的NPC和SC进行体外增殖试验和细胞因子谱分析。所有OVA喂养小鼠的肝脏在10天后将耐受性转移到OVA未处理小鼠。从高剂量OVA喂养的小鼠过继转移肝脏NPC的时间过程在24小时内转移了OVA耐受性;低剂量OVA喂养的小鼠需要大于或等于4天才能转移耐受性。在体外增殖反应的NPC显示，在两个剂量组的响应比controlgroup.Our结果表明，肝脏在诱导外周粘膜耐受模型中起着重要作用，特别是喂养高剂量的OVA。
Using a murine liver transplant model, we studied the liver's role in peripheral tolerance. Livers from BALB/c mice fed with ovalbumin (OVA) at either a low or high dose were transplanted into syngeneic recipients. Non-fed recipients were controls. Orthotopic liver transplantation (OLTx) was followed by OVA immunization and delayed-type hypersensitivity (DTH) challenge. The ex vivo adoptive transfer effect of liver nonparenchymal cells (NPCs) or spleen cells (SCs) from OVA-fed mice was examined. In vitro proliferative assays and cytokine profiles were conducted on NPCs and SCs from transplant recipients.Livers from all OVA-fed mice after 10 days transferred tolerance to OVA-naive mice. The time course of adoptive transfer of liver NPCs from high-dose OVA-fed mice transferred OVA tolerance within 24 h; low-dose OVA-fed mice required greater than or equal to 4 days to transfer tolerance. The in vitro proliferative response of the NPCs to OVA revealed a decreased response in both dosage groups over the control group.Our results suggest that the liver plays an important role in inducing peripheral tolerance in a mucosal tolerance model, especially feeding high-dose OVA.