NompC TRP channel required for vertebrate sensory hair cell mechanotransduction
NompC TRP channel required for vertebrate sensory hair cell mechanotransduction
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DOI：
10.1126/science.1084370
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发表时间：
2003-07-04
期刊：
影响因子：
56.9
通讯作者：
Nicolson, T
中科院分区：
文献类型：
--
作者：
Sidi, S;Friedrich, RW;Nicolson, T
脊椎动物的听觉和平衡感依赖于内耳的感觉毛细胞（HC）。HC的转导装置的中心元件是未知身份的机械门控离子通道。在这里，我们报告说，斑马鱼直系同源果蝇无机械受体电位C（nompC），它编码一个瞬时受体电位（TRP）通道，是至关重要的HC机械转导。在斑马鱼幼体中，nompC选择性地在感觉HC中表达。吗啉代介导的nompC功能的去除消除了转导依赖的内吞作用和HC中的电反应，导致幼虫耳聋和失衡。这些观察结果表明，nompC编码脊椎动物HC机械转导通道。
The senses of hearing and balance in vertebrates rely on the sensory hair cells (HCs) of the inner ear. The central element of the HC's transduction apparatus is a mechanically gated ion channel of unknown identity. Here we report that the zebrafish ortholog of Drosophila no mechanoreceptor potential C (nompC), which encodes a transient receptor potential (TRP) channel, is critical for HC mechanotransduction. In zebrafish larvae, nompC is selectively expressed in sensory HCs. Morpholino-mediated removal of nompC function eliminated transduction-dependent endocytosis and electrical responses in HCs, resulting in larval deafness and imbalance. These observations indicate that nompC encodes a vertebrate HC mechanotransduction channel.