IDENTIFICATION OF A TAP-DEPENDENT LEADER PEPTIDE RECOGNIZED BY ALLOREACTIVE T-CELLS SPECIFIC FOR A CLASS IB ANTIGEN
IDENTIFICATION OF A TAP-DEPENDENT LEADER PEPTIDE RECOGNIZED BY ALLOREACTIVE T-CELLS SPECIFIC FOR A CLASS IB ANTIGEN
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DOI：
10.1016/0092-8674(94)90550-9
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发表时间：
1994-11-18
期刊：
影响因子：
64.5
通讯作者：
FORMAN, J
中科院分区：
文献类型：
--
作者：
ALDRICH, CJ;DECLOUX, A;FORMAN, J
部分同种异体反应性细胞毒性T淋巴细胞(CTL)克隆识别Ib类抗原Qa-1需要靶细胞表达第二个基因，称为Qa-1决定簇修饰物(Qdm)。我们发现，Qdm与除D-k外的大多数D等位基因是相同的，并且来自D同种异体抗原的九聚体多肽可以恢复CTL。对缺乏QDM编码决定簇的细胞的识别。相同的D-k多肽在P3处有Ala--&Gt；Val交换，并且比Ala(P3)多肽需要大约4个对数的多肽才能裂解靶细胞。五个CTL克隆中的两个不依赖Qdm识别靶细胞，也识别Qdm多肽和Val(P3)变异体。虽然ADM多肽由3-11个残基组成，但它需要Tap转运蛋白才能表达。因此，对这种Ib类分子的反应提供了一种分析同种异体反应性以及抗原呈递途径的工具。
Recognition of the class Ib antigen Qa-1 by a portion of alloreactive cytotoxic T lymphocyte (CTL) clones requires that the target cell express a second gene, termed Qa-1 determinant modifier (Qdm). We show that Qdm is identical to most D allele genes, excepting D-k, and that a nonamer peptide derived from D alloantigens restores CTL. recognition on cells that lack the Qdm-encoded determinant. The equivalent D-k peptide has an Ala-->Val interchange at P3 and requires similar to 4 logs more peptide than the Ala(P3) peptide for target cell lysis. Two of five CTL clones, not dependent on Qdm for target cell recognition, also recognize the Qdm peptide as well as the Val(P3) variant. Although the adm peptide spans residues 3-11 from the leader, it requires the Tap transporters for its expression. Thus, the response against this class Ib molecule provides a tool for dissecting alloreactivity as well as pathways for antigen presentation.