Damage to the choroid plexus, ependyma and subependyma as a consequence of perinatal hypoxia/ischemia
Damage to the choroid plexus, ependyma and subependyma as a consequence of perinatal hypoxia/ischemia
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DOI：
10.1159/000069052
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发表时间：
2002-09-01
影响因子：
2.9
通讯作者：
Levison, SW
中科院分区：
文献类型：
--
作者：
Rothstein, RP;Levison, SW
早产儿脑缺血缺氧（H/I）是脑性瘫痪和智力低下的主要原因。这种损伤的最终结果的一个重要决定因素是大脑中的干细胞和祖细胞受到影响的程度。对这些细胞的不可逆损伤将损害婴儿大脑的正常发育，从而损害其功能。在本研究中，我们检查了H/I后的早期间隔，以确定室周区域中的哪些细胞最脆弱。在从围产期H/I损伤恢复0小时时，脉络丛显示广泛的坏死性损伤。邻近的室管膜和室管膜下细胞也受到影响。观察到室管膜和内侧室管膜下细胞肿胀;然而，这些细胞很少受到永久性损伤。相比之下，室管膜下区（SVZ）最外侧的细胞显示出更多延迟的，但广泛的凋亡和杂交细胞死亡。有趣的是，在肿胀的室管膜细胞附近以及受影响的室管膜下观察到活化的巨噬细胞/小胶质细胞。我们的结论是，脉络丛是一个特别脆弱的结构，在未成熟的大脑，而室管膜和相邻的室管膜下细胞是相对抵抗损伤。由于SVZ的内侧部分含有神经干细胞，我们预测神经干细胞将特别抵抗围产期H/I脑损伤。版权所有（C）2002 S. Karger AG，巴塞尔。
Cerebral hypoxia/ischemia (H/I) of the premature infant is a major cause of cerebral palsy and mental retardation. An important determinant of the ultimate outcome from this insult is the extent to which the stem cells and progenitors in the brain are affected. Irreversible injury to these cells will impair normal development of the infant's brain and, hence, its function. In the present study, we examine early intervals after H/I to identify which cells in the periventricular region are most vulnerable. At 0 h of recovery from a perinatal H/I insult, the choroid plexus shows extensive necrotic damage. The adjacent ependymal and subependymal cells are also affected. Swelling of the ependymal and medial subependymal cells is observed; however, these cells rarely sustain permanent damage. By contrast, cells in the most lateral aspect of the subventricular zone (SVZ) show more delayed, but extensive apoptotic and hybrid cell deaths. Interestingly, activated macrophages/microglia are observed adjacent to the swollen ependymal cells as well as within the affected subependyma. We conclude that the choroid plexus is an especially vulnerable structure in the immature brain, whereas the ependymal and adjacent subependymal cells are relatively resistant to damage. As the medial aspect of the SVZ contains neural stem cells, we predict that neural stem cells will be especially resistant to perinatal H/I brain damage. Copyright (C) 2002 S. Karger AG, Basel.