In vivo real-time visualization of tissue blood flow and angiogenesis using Ag2S quantum dots in the NIR-II window
In vivo real-time visualization of tissue blood flow and angiogenesis using Ag2S quantum dots in the NIR-II window
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在 NIR-II 窗口中使用 Ag2S 量子点对组织血流和血管生成进行体内实时可视化
DOI：
10.1016/j.biomaterials.2013.10.010
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发表时间：
2014-01-01
期刊：
影响因子：
14
通讯作者：
Wang, Qiangbin
中科院分区：
文献类型：
--
作者：
Li, Chunyan;Zhang, Yejun;Wang, Qiangbin
由于活体组织的散射和吸收以及内源性自发荧光的存在，提高荧光纳米探针的组织穿透深度和空间分辨率仍然是其在活体成像中的实际应用面临的巨大挑战。在这里，我们提出，Ag 2S量子点（QDs），不含有毒离子，表现出长的循环时间和高稳定性，作为一种新的荧光探针在第二近红外窗口（NIR-II，1000-1350 nm），使在体内监测淋巴引流和血管网络与深组织渗透和高的空间和时间分辨率。此外，NIR-II荧光成像与Ag 2S量子点提供的空间分辨率（类似于40 μ m），使我们能够跟踪血管生成介导的微小肿瘤（直径2-3毫米）在体内。我们的研究结果表明，Ag 2S量子点是很有前途的NIR-II荧光纳米探针，可用于外科治疗，如前哨淋巴结（SLN）清扫，以及评估组织和器官的血液供应和筛选抗血管生成药物。(C)2013爱思唯尔有限公司保留所有权利。
Improving the tissue penetration depth and spatial resolution of fluorescence-based optical nanoprobes remains a grand challenge for their practical applications in in vivo imaging, due to the scattering and absorption and endogenous autofluorescence of living tissues. Here, we present that Ag2S quantum dots (QDs), containing no toxic ions, exhibiting long circulation time and high stability, act as a new kind of fluorescent probes in the second near-infrared window (NIR-II, 1000-1350 nm) which enable in vivo monitoring of lymphatic drainage and vascular networks with deep tissue penetration and high spatial and temporal resolution. In addition, NIR-II fluorescence imaging with Ag2S QDs provide ultrahigh spatial resolution (similar to 40 mu m) that permits us to track angiogenesis mediated by a tiny tumor (2-3 mm in diameter) in vivo. Our results indicate that Ag2S QDs are promising NIR-II fluorescent nanoprobes that could be useful in surgical treatments such as sentinel lymph node (SLN) dissection as well in assessment of blood supply in tissues and organs and screening of anti-angiogenic drugs. (C) 2013 Elsevier Ltd. All rights reserved.