Polyclonal activation of the murine immune system by an antibody to IgD V. Effect on germinal centers.
Polyclonal activation of the murine immune system by an antibody to IgD V. Effect on germinal centers.
复制标题
IgD V 抗体对小鼠免疫系统的多克隆激活。对生发中心的影响。
DOI：
10.1002/eji.1830140810
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发表时间：
1984
影响因子：
5.4
通讯作者：
Thorbecke,GJ
中科院分区：
文献类型：
--
作者：
Flotte,TJ;Finkelman,FD;Thorbecke,GJ
Heterologous anti‐mouse δ chain has been shown to induce T‐independent polyclonal B cell proliferation by 24 h after injection into mice and a T‐dependent polyclonal increase in the number of IgG‐secreting cells in spleen and lymph nodes 6 days after injection. The effect of anti‐δ on germinal center cells has been difficult to determine, however, since anti‐δ‐induced blast transformation of mantle zone cells makes it difficult to distinguish these cells from germinal center cells. To clarify the effect of anti‐δ on germinal centers, we stained lymph node sections from control and anti‐δ‐injected mice with peanut agglutinin conjugated to horseradish peroxidase which clearly differentiates the germinal center cells from other large and small lymphoid cells.For the first 5 days after i.v. injection of goat anti‐mouse δ, the germinal center volumes did not differ significantly from those of mice injected with control antibodies. On days 6 and 7 after injection, the lymph node germinal center volumes of anti‐δ‐treated mice increased on average over 10‐fold as compared to those of mice injected with an equal amount of control antibody. Thus, while anti‐δ antibody directly induced blastogenesis of mantle layer B cells, it appears to have little direct effect on germinal center cells. In contrast, rapid germinal center development is seen in anti‐δ‐injected mice during the T‐dependent phase of B cell activation and occurs simultaneously with the appearance of large numbers of IgG‐secreting cells.
