Identification and characterization of mouse PSF1-binding protein, SLD5
Identification and characterization of mouse PSF1-binding protein, SLD5
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DOI：
10.1016/j.bbrc.2005.11.136
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发表时间：
2006-01-27
影响因子：
3.1
通讯作者：
Takakura, N
中科院分区：
文献类型：
--
作者：
Kong, LY;Ueno, M;Takakura, N
虽然大多数体细胞不能增殖，但未成熟细胞不断增殖以产生成熟细胞。最近，我们从小鼠造血干细胞特异性cDNA文库中克隆了PSF1，并报道PSF1对未成熟细胞的增殖是不可缺少的。为了鉴定PSF1结合蛋白，我们利用酵母双杂交系统以PSF1为诱饵，鉴定并克隆了SLD5。SLD5与PSF1的中心区域相互作用。SLD 5的组织分布与PSF 1非常相似。当过表达时，SLD5蛋白与PSF1共定位。这些数据表明，PSF1和SLD5可能在未成熟细胞群的增殖中合作。(c)2005年爱思唯尔公司All rights reserved.
Although most somatic cells cannot proliferate, immature cells proliferate continuously to produce mature cells. Recently, we cloned mouse PSF1 from a hematopoietic stem cell specific cDNA library and reported that PSF1 is indispensable for the proliferation of immature cells. To identify the PSF1-binding protein, we used the yeast two-hybrid system with PSF1 as bait, and identified and cloned SLD5. SLD5 interacted with a central region of PSF1. Tissue distribution of SLD5 was quite similar to that of PSF1. When overexpressed, SLD5 protein was co-localized with PSF1. These data suggest that PSF1 and SLD5 may cooperate in the proliferation of immature cell populations. (c) 2005 Elsevier Inc. All rights reserved.