Control of the rubber anthracnose fungusColletotrichum gloeosporioidesusing culture filtrate extract fromStreptomyces deccanensisQY-3
Control of the rubber anthracnose fungusColletotrichum gloeosporioidesusing culture filtrate extract fromStreptomyces deccanensisQY-3
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利用德卡链霉菌 QY-3 培养滤液提取物防治橡胶炭疽病菌胶孢炭疽病
DOI：
10.1007/s10482-020-01465-8
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发表时间：
2020-08-19
影响因子：
2.6
通讯作者：
Liu, Zhiqiang
中科院分区：
文献类型：
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作者：
Gu, Liushuang;Zhang, Kai;Liu, Zhiqiang
炭疽病是橡胶炭疽病的主要病原，给天然橡胶工业造成巨大损失。本研究分离到一株具有良好拮抗活性的放线菌菌株QY-3。类胶孢子菌，抑制率为86.6%。经初步鉴定，菌株QY-3为十冠链霉菌。筛选出小米培养基产抗真菌代谢物的最佳发酵液。去扫描QY-3。小米发酵液的培养滤液(CFE)。QY-3对植物病原真菌具有广谱抗真菌活性，其EC(50)抑制菌丝生长。类胶孢子菌6.3微克/毫升。CFE具有良好的热稳定性和pH稳定性，对菌丝有破碎作用，对分生孢子萌发有抑制作用。球孢子菌属。100 mU g/mLCFE对橡胶炭疽病有明显的防治效果，接种后第5天的防效为63.7%。两个对NC有抑制作用的化合物。从CFE中分离得到类球孢子菌素、邻氨基苯甲酸和生根霉素。两种化合物对C。类胶孢子菌分别为29.3和23.0微克/毫升。总而言之，CFE来自。QY-3是一种很有潜力的橡胶炭疽病杀菌剂。
Colletotrichum gloeosporioidesis a main cause of rubber anthracnose, which results in a huge loss for the natural rubber industry. In this study, an actinomycete strain QY-3 was isolated and had good antagonistic activity againstC. gloeosporioideswith an inhibition rate of 86.6%. Strain QY-3 was identified as Streptomyces deccanensispreliminarily. Millet medium was selected as the optimal fermentation broth for antifungal metabolites production byS. deccanensisQY-3. The culture filtrate extract (CFE) from the millet broth ofS. deccanensisQY-3 exhibits broad-spectrum antifungal activity against plant pathogenic fungi, and its EC(50)inhibiting the mycelial growth ofC. gloeosporioidesis 6.3 mu g/mL. The CFE has good thermal and pH stabilities, and it can break the hyphae and inhibit the conidial germination ofC. gloeosporioides. 100 mu g/mL of CFE had an obvious control effect on rubber anthracnose, and the control efficacy was 63.7% on 5 days after inoculation. Two compounds with inhibitory effects onC. gloeosporioides, anthranilic acid and sangivamycin, were isolated from the CFE. The MICs of both compounds againstC. gloeosporioideswere 29.3 and 23.0 mu g/mL respectively. In conclusion, the CFE fromS. deccanensisQY-3 has great potential to be a promising fungicide for rubber anthracnose.