Tissue‐specific isoforms of the bovine mitochondrial ATP synthase γ‐subunit
Tissue‐specific isoforms of the bovine mitochondrial ATP synthase γ‐subunit
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牛线粒体 ATP 合酶 γ 亚基的组织特异性亚型
DOI：
10.1016/0014-5793(93)81089-i
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发表时间：
1993
期刊：
影响因子：
3.5
通讯作者：
Y. Kagawa
中科院分区：
文献类型：
--
作者：
C. Matsuda;H. Endo;H. Hirata;H. Morosawa;M. Nakanishi;Y. Kagawa
首次鉴定了牛F0F1 - ATP合成酶γ -亚基的组织特异性亚型。这两种异构体，心脏型和肝脏型，是通过选择性剪接产生的，肝脏型RNA转录物包含37个核苷酸序列作为盒式外显子。F1γ -亚基的两个亚型的C端片段的蛋白质测序表明，肝型亚型在C端有额外的天冬氨酸残基，而心脏型亚型不存在。
Tissue‐specific isoforms of the γ‐subunit of the bovine F0F1‐ATP synthase were identified for the first time. The two isoforms, heart and liver type, were generated by alternative splicing, the liver‐type RNA transcript containing a 37‐nucleotide sequence as a cassette exon. Protein sequencing of the C‐terminal fragments of the two isoforms of the F1γ‐subunit indicated that the liver‐type isoform had an additional aspartate residue at the C terminus, not present in the heart type one.