Urinary MicroRNA-30c-5p and MicroRNA-192-5p as potential biomarkers of ischemia-reperfusion-induced kidney injury
Urinary MicroRNA-30c-5p and MicroRNA-192-5p as potential biomarkers of ischemia-reperfusion-induced kidney injury
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尿液 MicroRNA-30c-5p 和 MicroRNA-192-5p 作为缺血再灌注肾损伤的潜在生物标志物
DOI：
10.1177/1535370216685005
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发表时间：
2017-03-01
影响因子：
3.2
通讯作者：
Zhang, Wen
中科院分区：
文献类型：
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作者：
Zou, Yan-Fang;Wen, Dan;Zhang, Wen
由于缺乏已知的生物标志物，急性肾损伤的早期检测是困难的;先前的研究试图鉴定用于在早期检测急性肾损伤的新生物标志物。MicroRNA是一种21 - 23个核苷酸的非编码RNA分子，已成为各种疾病诊断和预后的理想标志物。本研究的目的是确定microRNA在缺血再灌注肾损伤中的表达谱，并确定将候选microRNA作为检测I/R肾损伤的生物标志物的可能性。在建立大鼠I/R肾损伤模型的基础上，对手术开始时（0 h）和手术后72 h的大鼠尿液进行微阵列分析。为了验证结果，收集了71名接受心脏手术的患者的尿液样本，然后进行尿液分析以确定microRNA浓度。在大鼠尿液中检测到microRNA的另一种表达谱。microRNA定量验证结果显示，术后尿中miR-30c-5p、miR-192 - 5p和miR-378a-3p的表达明显升高，与芯片分析结果一致，且早于肾损伤分子-1（KIM-1）。在急性肾损伤患者中，术后2 h也检测到miR-30c-5p和miR-192 - 5p水平升高，与基于蛋白质的生物标志物相比，miR-30c-5p显示出更好的诊断价值。总之，基于所建立的缺血-再灌注动物模型，在大鼠尿液中检测到异常的microRNA表达谱。miR-30c-5p和miR-192 - 5p均为I/R诱导的肾损伤的重要潜在诊断标志物。
Early detection of acute kidney injury is difficult due to lack of known biomarkers; previous studies have tried to identify new biomarkers for detecting acute kidney injury at an early stage. MicroRNA, a 21-23 nucleotide noncoding RNA molecule, has emerged as a desirable marker in the diagnosis and prognosis of various diseases. This study aims to identify the expression profile of microRNA in ischemia-reperfusion-induced kidney injury and determine the possibility of using the candidate microRNA as biomarker for the detection of I/R-induced kidney injury. Based on the established rat model of I/R-induced kidney injury, a microarray analysis of rat urine was performed at the beginning of operation (0 h) as well as 72 h post operation. To validate the results, urine samples from 71 patients who underwent cardiac surgery were collected, after which urinalysis was conducted to determine the microRNA concentration. An alternative expression profile of microRNAs was detected in rat urine. The quantitative validation of microRNA showed that the expression of miR-30c-5p, miR-192-5p, and miR-378a-3p was elevated significantly in urine post operation, which was consistent with those of the microarray analysis and earlier than kidney injury molecule-1 (KIM-1). In patients with acute kidney injury, increased levels of miR-30c-5p and miR-192-5p were also detected 2 h post operation, and miR-30c-5p showed preferable diagnostic value compared with protein-based biomarkers. In conclusion, an aberrant expression profile of microRNA was detected in rat urine based on the established ischemia-reperfusion animal model. Both miR-30c-5p and miR-192-5p served as important potential diagnostic markers for I/R-induced kidney injury.