Time-course study of histological and genetic patterns of differentiation in human engineered oral mucosa
Time-course study of histological and genetic patterns of differentiation in human engineered oral mucosa
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DOI：
10.1002/term.38
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发表时间：
2007-09-01
影响因子：
3.3
通讯作者：
Campos, A.
中科院分区：
文献类型：
--
作者：
Alaminos, M.;Garzon, I.;Campos, A.
缺乏足够的口腔粘膜可用于口内移植是一个主要的外科问题，需要新的口腔组织来源用于临床。在这方面，一些模型的工程化口腔粘膜已报告的日期，但很少有人知道的结构和遗传机制，发生在这些组织替代品的发展和成熟的过程中。我们已经进行了一个时间过程中的基因和形态学模式的细胞和组织分化，在口腔粘膜构建后3，7，11和21天的发展研究。我们的电子显微镜和微阵列分析表明，组织工程产生的口腔粘膜结构经历了一个渐进的细胞分化过程，依次形成和成熟的几层上皮（表达分层蛋白、sciellin、外皮蛋白、透明质酸和激肽释放酶7），细胞间连接（具有斑嗜蛋白、桥粒胶原蛋白、桥粒芯糖蛋白和钙粘蛋白的表达）、细胞角蛋白、基底膜（层粘连蛋白、胶原M和细胞外基质（双糖链蛋白聚糖、基质金属蛋白酶）。总之，尽管体外形成的角化水平和类型可能不同，但口腔粘膜替代物与天然组织非常相似。版权所有（c）2007约翰威利父子有限公司。
The lack of sufficient oral mucosa available for intra-oral grafting is a major surgical problem, and new sources of oral tissues for clinical use are needed. In this regard, some models of engineered oral mucosa have been reported to date, but little is known about the structural and genetic mechanisms that occur during the process of development and maturation of these tissue substitutes. We have carried out a time-course study of the genes and morphological patterns of cell and tissue differentiation that develop in oral mucosa constructs after 3, 7, 11 and 21 days of development. Our electron microscopy and microarray analyses demonstrated that the oral mucosa constructs generated by tissue engineering undergo a progressive process of cell differentiation with the sequential formation and maturation of several layers of epithelium (with expression of stratifin, sciellin, involucrin, trichohyalin and kallikrein 7), intercellular junctions (with expression of plakophilin, desmocollin, desmoglein and cadherins), cytokeratins, a basement membrane (laminins, collagen M and the extracellular matrix (biglycan, matrix metalloproteinases). In conclusion, although the level and type of keratinization developed in vitro could be different, the oral mucosa substitutes were very similar to the native tissues. Copyright (c) 2007 John Wiley & Sons, Ltd.