The primer generator: A program that facilitates the selection of oligonucleotides for site-directed mutagenesis
The primer generator: A program that facilitates the selection of oligonucleotides for site-directed mutagenesis
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DOI：
10.2144/99264st02
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发表时间：
1999-04-01
期刊：
影响因子：
2.7
通讯作者：
Lawler, JF
中科院分区：
文献类型：
--
作者：
Turchin, A;Lawler, JF
定点突变是一种强有力的工具，使分子生物学家能够对改变的核酸和蛋白质进行功能分析。较新的基于PCR的诱变技术已经将诱变过程缩短到一天。虽然每种技术都有其优点，但都需要一种策略来从含有诱变和野生型基因的群体中分离所需的克隆。在这份报告中，我们描述了一个基于万维网的计算机程序，便于诱变引物的设计，这样成功诱变的克隆可以通过一个独特的限制性位点的存在或不存在进行鉴定。
Site-directed mutagenesis is a powerful tool that has enabled molecular biologists to perform functional analysis of altered nucleic acids and proteins. Newer er PCR-based mutagenesis techniques have reduced the process of mutagenesis to as little as one day. While each technique has its advantages, both require a strategy to isolate the desired clone from a population that contains mutagenized and wild-type genes. In this report, we describe a World Wide Web-based computer program that facilitates the design of mutagenic primers such that successfully mutagenized clones can be identified by the presence or absence of a unique restriction site.