Microfabricated polymer chip for capillary gel electrophoresis
Microfabricated polymer chip for capillary gel electrophoresis
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DOI：
10.1021/bp010075m
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发表时间：
2001-09-01
影响因子：
2.9
通讯作者：
Endo, I
中科院分区：
文献类型：
--
作者：
Hong, JW;Hosokawa, K;Endo, I
提出了一种用于毛细管凝胶电泳的聚合物（PDMS：聚二甲基硅氧烷）微芯片，该芯片可以在小实验规模下分离不同大小的DNA分子。这种微芯片可以通过简单的PDMS成型方法轻松生产，而无需使用复杂的粘合过程。与传统的玻璃、石英或硅制成的微芯片不同，这种PDMS微芯片可以作为一次性设备使用。芯片上的毛细管通道部分填充琼脂糖凝胶，可以提高不同大小DNA分子的分离分辨率，与自由流动或聚合物溶液形式的毛细管电泳相比，可以缩短样品分离所需的通道长度。我们讨论了用于微通道的凝胶制备的最佳条件。DNA分子在电场驱动下成功分离，在2.0%琼脂糖填充、有效分离长度为8 mm的微通道中形成100 bp ~ 1 kbp的条带。
A polymer (PDMS: poly(dimethylsiloxane)) microchip for capillary gel electrophoresis that can separate different sizes of DNA molecules in a small experimental scale is presented. This microchip can be easily produced by a simple PDMS molding method against a microfabricated master without the use of elaborate bonding processes. This PDMS microchip could be used as a single use device unlike conventional microchips made of glass, quartz or silicon. The capillary channel on the chip was partially filled with agarose gel that can enhance separation resolution of different sizes of DNA molecules and can shorten the channel length required for the separation of the sample compared to capillary electrophoresis in free-flow or polymer solution format. We discuss the optimal conditions for the gel preparation that could be used in the microchannel. DNA molecules were successfully driven by an electric field and separated to form bands in the range of 100 bp to 1 kbp in a 2.0% agarose-filled microchannel with 8 mm of effective separation length.