Mating activates neuroendocrine pathways signaling hunger in Drosophila females.
Mating activates neuroendocrine pathways signaling hunger in Drosophila females.
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DOI：
10.7554/elife.85117
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发表时间：
2023-05-15
期刊：
影响因子：
7.7
通讯作者：
Scott K
中科院分区：
文献类型：
--
作者：
Laturney M;Sterne GR;Scott K
交配的雌性重新分配资源给后代生产，导致营养需求的变化和能量稳态的挑战。虽然观察到跨物种，调节交配雌性的营养需求的神经和内分泌机制还没有很好的理解。在这里，我们发现，交配果蝇雌性增加糖的摄入量，这是由性二型胰岛素受体（LGR 3）神经元的活动。在处女中，Lgr 3+细胞的活性降低，因为它们从活跃的雌性特异性pCd-2细胞接收抑制性输入，限制糖的摄入。在交配过程中，雄性存款性肽进入雌性生殖道，这沉默了一个三级交配状态电路，并启动了雌性交配后的反应。我们发现，pCd-2神经元也成为沉默后，由于交配状态电路的直接突触输入交配。因此，在交配雌性pCd-2抑制减弱，激活下游Lgr 3+神经元，促进糖的摄入。该电路将配对信号转换为长期饥饿信号。我们的研究结果表明，交配电路改变营养感应中心，以增加喂养交配的女性，提供了一种机制，以增加摄入量的预期与繁殖相关的能量成本。
Mated females reallocate resources to offspring production, causing changes to nutritional requirements and challenges to energy homeostasis. Although observed across species, the neural and endocrine mechanisms that regulate the nutritional needs of mated females are not well understood. Here, we find that mated Drosophila melanogaster females increase sugar intake, which is regulated by the activity of sexually dimorphic insulin receptor (Lgr3) neurons. In virgins, Lgr3+ cells have reduced activity as they receive inhibitory input from active, female-specific pCd-2 cells, restricting sugar intake. During copulation, males deposit sex peptide into the female reproductive tract, which silences a three-tier mating status circuit and initiates the female postmating response. We show that pCd-2 neurons also become silenced after mating due to the direct synaptic input from the mating status circuit. Thus, in mated females pCd-2 inhibition is attenuated, activating downstream Lgr3+ neurons and promoting sugar intake. Together, this circuit transforms the mated signal into a long-term hunger signal. Our results demonstrate that the mating circuit alters nutrient sensing centers to increase feeding in mated females, providing a mechanism to increase intake in anticipation of the energetic costs associated with reproduction.