INTERACTION OF PLASMA-MEMBRANE FIBRONECTIN RECEPTOR WITH TALIN - A TRANSMEMBRANE LINKAGE
INTERACTION OF PLASMA-MEMBRANE FIBRONECTIN RECEPTOR WITH TALIN - A TRANSMEMBRANE LINKAGE
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DOI：
10.1038/320531a0
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发表时间：
1986-04-10
期刊：
影响因子：
64.8
通讯作者：
BURRIDGE, K
中科院分区：
文献类型：
--
作者：
HORWITZ, A;DUGGAN, K;BURRIDGE, K
许多观察结果表明细胞骨架和细胞外基质之间存在跨膜连接。在成纤维细胞中，光镜和电镜观察揭示了细胞表面的肌动蛋白丝和细胞外纤连蛋白1 -3之间的协同排列。这些关联在细胞基质相互作用的位点被观察到，通常沿着应力纤维，有时这些微丝束终止于粘附斑（局灶性接触）。非形态学证据也表明细胞骨架和细胞外基质之间的功能联系。向转化细胞中加入纤连蛋白可诱导细胞变平和肌动蛋白细胞骨架重组，并伴随出现应力细胞阵列4 -6。相反，用细胞松弛素B处理破坏肌动蛋白细胞骨架导致纤连蛋白从细胞表面释放7。到目前为止，还没有关于这种跨膜连接所涉及的分子的详细知识，尽管已经提出了几种蛋白质作为肌动蛋白丝束和质膜的细胞质面之间的附着链的候选者：这些包括黏着斑蛋白8、α-辅肌动蛋白9和talin 10，每一种都在肌动蛋白丝束与质膜和下面的细胞表面纤连蛋白相互作用的区域被鉴定10 -13。最近，细胞基质附着（CSAT）抗原14已被确定为纤维连接蛋白的质膜受体15，提高了这种糖蛋白复合物可能作为纤维连接蛋白和一个或多个提到的潜在细胞骨架成分之间的桥梁的可能性。在这里，我们已经研究了纯化的CSAT抗原与这些细胞骨架成分的相互作用，我们证明了CSAT抗原和塔林之间的相互作用。
Many observations suggest the presence of transmembrane linkages between the cytoskeleton and the extracellular matrix. In fibroblasts both light and electron microscopic observations reveal a co-alignment between actin filaments at the cell surface and extracellular fibronectin1–3. These associations are seen at sites of cell matrix interaction, frequently along stress fibres and sometimes where these bundles of microfilaments terminate at adhesion plaques (focal contacts). Non-morphological evidence also indicates a functional linkage between the cytoskeleton and extracellular matrix. Addition of fibronectin to transformed cells induces flattening of the cells and a reorganization of the actin cytoskeleton, with the concomitant appearance of arrays of stress fibres4–6. Conversely, disruption of the actin cytoskeleton by treatment with cytochalasin B leads to release of fibronectin from the cell surface7. As yet, there is no detailed knowledge of the molecules involved in this transmembrane linkage, although several proteins have been suggested as candidates in the chain of attachment between bundles of actin filaments and the cytoplasmic face of the plasma membrane: these include vinculin8,α-actinin9and talin10, each one having been identified at regions where bundles of actin filaments interact with the plasma membrane and underlying cell-surface fibronectin10–13. Recently, the cell-substrate attachment (CSAT) antigen14has been identified as a plasma membrane receptor for fibronectin15, raising the possibility that this glycoprotein complex may serve as a bridge between fibronectin and one or more of the underlying cytoskeletal components mentioned. Here we have investigated the interaction of the purified CSAT antigen with these cytoskeletal components, and we demonstrate an interaction specifically between the CSAT antigen and talin.