Tumor protein D54 defines a new class of intracellular transport vesicles
Tumor protein D54 defines a new class of intracellular transport vesicles
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DOI：
10.1083/jcb.201812044
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发表时间：
2020-01-06
影响因子：
7.8
通讯作者：
Royle, Stephen J.
中科院分区：
文献类型：
--
作者：
Larocque, Gabrielle;La-Borde, Penelope J.;Royle, Stephen J.
蛋白质和脂质从一个膜隔室到另一个膜隔室的转运是通过胞内囊泡。我们研究了肿瘤蛋白D54（TPD 54/TPD 52 L2）的功能，发现TPD 54参与多种膜运输途径：顺行运输、再循环和高尔基体完整性。为了了解TPD 54如何控制这些不同的功能，我们使用了一种诱导方法将TPD 54重新路由到线粒体。令人惊讶的是，这种操作导致在线粒体表面捕获许多小囊泡（直径30 nm）。超分辨率成像证实了在正常条件下存在类似大小的TPD 54阳性结构。TPD 54似乎定义了一类新的运输囊泡，我们称之为细胞内纳米囊泡（INVs）。INV满足三个功能标准。它们含有特定的货物，它们具有某些用于融合的R-SNARE，并且它们被赋予多种Rab GTP酶（43种测试中的16种）。INVs的分子异质性和TPD 54的多样性功能表明INVs具有不同的膜起源和许多目的地。我们认为INVs是一类运输囊泡，在这些不同的位置之间转移货物。
Transport of proteins and lipids from one membrane compartment to another is via intracellular vesicles. We investigated the function of tumor protein D54 (TPD54/TPD52L2) and found that TPD54 was involved in multiple membrane trafficking pathways: anterograde traffic, recycling, and Golgi integrity. To understand how TPD54 controls these diverse functions, we used an inducible method to reroute TPD54 to mitochondria. Surprisingly, this manipulation resulted in the capture of many small vesicles (30 nm diameter) at the mitochondrial surface. Super-resolution imaging confirmed the presence of similarly sized TPD54-positive structures under normal conditions. It appears that TPD54 defines a new class of transport vesicle, which we term intracellular nanovesicles (INVs). INVs meet three criteria for functionality. They contain specific cargo, they have certain R-SNAREs for fusion, and they are endowed with a variety of Rab GTPases (16 out of 43 tested). The molecular heterogeneity of INVs and the diverse functions of TPD54 suggest that INVs have various membrane origins and a number of destinations. We propose that INVs are a generic class of transport vesicle that transfer cargo between these varied locations.