Human apolipoprotein E receptor 2 - A novel lipoprotein receptor of the low density lipoprotein receptor family predominantly expressed in brain
Human apolipoprotein E receptor 2 - A novel lipoprotein receptor of the low density lipoprotein receptor family predominantly expressed in brain
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DOI：
10.1074/jbc.271.14.8373
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发表时间：
1996-04-05
影响因子：
4.8
通讯作者：
Yamamoto, T
中科院分区：
文献类型：
--
作者：
Kim, DH;Iijima, H;Yamamoto, T
分离和鉴定了一种新的脂蛋白受体，命名为载脂蛋白E受体2(ApoER2)。新的受体由五个功能结构域组成，类似于低密度脂蛋白(LDL)和极低密度脂蛋白(VLDL)受体。低密度脂蛋白受体缺陷的中国仓鼠卵巢细胞表达人载脂蛋白ER2结合富含载脂蛋白E的β迁移性极低密度脂蛋白，具有高亲和力和内在化。低密度脂蛋白与这些细胞的亲和力要低得多。该受体的4.5kb和8.5kb的mRNAs在人脑和胎盘中表达最高。在兔组织中，4、4.5、5.5、8.5和11kb的多种mRNA在脑和睾丸中表达最强，在卵巢中表达最少，在其他组织中检测不到。在大鼠肾上腺嗜铬细胞瘤PC12细胞中，神经生长因子可诱导其受体mRNA的表达。原位杂交显示，受体转录本在小脑皮质、脉络丛、室管膜、海马体、嗅球中表达最强，在大脑皮层中表达最少。在小脑皮质，受体转录物密集分布于浦肯野细胞胞体。
Isolation and characterization of a human cDNA demonstrated a novel lipoprotein receptor designated apolipoprotein E receptor 2 (apoER2). The new receptor consists of five functional domains resembling the low density lipoprotein (LDL) and very low density lipoprotein (VLDL) receptors. LDL receptor deficient Chinese hamster ovary cells expressing human apoER2 bound apoE rich beta-migrating VLDL with high affinity and internalized. LDL was bound with much lower affinity to these cells. The 4.5- and 8.5-kb mRNAs for the receptor were most highly expressed in human brain and placenta. In rabbit tissues, multiple species of the mRNA with 4, 4.5, 5.5, 8.5, and 11 kb were detected most intensely in brain and testis and, to a much lesser extent, in ovary, but were undetectable in other tissues. In rat adrenal pheochromocytoma PC12 cells, the receptor mRNA was induced by treatment of the cells with nerve growth factor. The receptor transcripts were detectable most intensely in the cerebellar cortex, choroid plexus, ependyma, hippocampus, olfactory bulb and, to a much lesser extent, in the cerebral cortex as revealed by in situ hybridization histochemistry. In the cerebellar cortex, the receptor transcripts were densely deposited in Purkinje cell somata.