Cytoplasmic streaming in internodal cells ofNitella under centrifugal acceleration: a study done with a newly constructed centrifuge microscope
Cytoplasmic streaming in internodal cells ofNitella under centrifugal acceleration: a study done with a newly constructed centrifuge microscope
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离心加速下尼泰拉节间细胞的细胞质流动：用新构建的离心显微镜进行的研究
DOI：
10.1007/bf01323074
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发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
M. Kikuyama
中科院分区：
文献类型：
--
作者：
E. Kamitsubo;Y. Ohashi;M. Kikuyama
我们构造了一种新的频闪式离心显微镜，用它研究了离心加速度作用下的丽藻结间细胞胞质流动。在中等离心加速度（ca. 50-100×g），节间细胞胞质流动方向与皮层下纤维方向平行。适度的离心加速度既不加速也不减慢邻近皮层下纤维流动的内质的速度。然而，内质流在电刺激后突然停止。在离心加速度为50-100×g，甚至900× g的情况下，当电刺激引起血流停止时，邻近皮层下原纤维的内质网被暂时固定。有人建议，在流动停止时，形成的固定内质网和皮层下的原纤维之间的暂时的交叉桥梁。大量的内质作为一个整体，在平行于皮层下纤维的方向流动，并立即停止电刺激。停止后不久，大量内质开始被动地在平行于离心加速度的方向上流动，这是离心力的结果。
We constructed a new centrifuge microscope of the stroboscopic type, with which the cytoplasmic streaming inNitellainternodal cells under centrifugal acceleration was studied. Under moderate centrifugal acceleration (ca. 50–100×g), the direction of cytoplasmic streaming in an internodal cell ofNitellais parallel to the direction of the subcortical fibrils. The speed of endoplasm flowing contiguous to the subcortical fibrils is neither accelerated nor retarded by moderate centrifugal acceleration. The endoplasmic flow, however, stops suddenly following an electrical stimulus. The endoplasm contiguous to the subcortical fibrils is immobilized transiently at the time of streaming cessation induced by an electrical stimulus under centrifugal acceleration at 50–100×g, even at 900×g. It is suggested that transitory cross bridges between the immobilized endoplasm and the subcortical fibrils are formed at the time of streaming cessation. The bulk endoplasm flows as a whole in the direction parallel to that of the subcortical fibrils and stops promptly upon electrical stimulation. Soon after the stoppage the bulk endoplasm starts to flow passively in the direction parallel to that of the centrifugal acceleration as a result of the centrifugal force.