Screening of miRNAs in human follicular fluid reveals an inverse relationship between microRNA-663b expression and blastocyst formation
Screening of miRNAs in human follicular fluid reveals an inverse relationship between microRNA-663b expression and blastocyst formation
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人类卵泡液中 miRNA 的筛选揭示了 microRNA-663b 表达与囊胚形成之间的负相关关系
DOI：
10.1016/j.rbmo.2018.03.021
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发表时间：
2018-07-01
影响因子：
4
通讯作者：
Sun, Xiao-xi
中科院分区：
文献类型：
--
作者：
Fu, Jing;Qu, Rong-gui;Sun, Xiao-xi
研究问题：卵泡液中发现的miRNA是否与相应卵母细胞的胚泡形成相关？设计图：在这项研究中，收集了91名妇女的含有成熟卵母细胞的单个卵泡的91份卵泡液样本，并将其分为具有活胚泡的组1（n = 38）和无胚泡的组2（n = 53）。采用TaqMan人类miRNA卡片和定量逆转录聚合酶链反应（RT-PCR）鉴定两组卵泡液中差异表达的miRNA。我们发现MIR-663 B在产生活胚泡的卵母细胞的卵泡液中与那些没有发育成胚泡的卵母细胞的卵泡液中显著差异表达（14.16 +/- 7.00对23.68 +/- 17.02; P = 0.019），以及发育成形态良好的囊胚与形态不良的囊胚（11.69 +/- 3.49 vs 20.16 +/- 9.33; P = 0.003）。MIR-人卵泡液中663 B表达水平与囊胚形成呈显著负相关，可作为胚胎发育的客观评价指标IVF后的发展潜力(C)2018生殖保健有限公司由爱思唯尔有限公司出版。版权所有。
Research Question: Are miRNAs found in follicular fluid related to blastocyst formation from the corresponding oocytes?Design: In this study, 91 individual follicular fluid samples from single follicles containing mature oocytes from 91 women were collected and classified into group 1 (n = 38) with viable blastocysts, and group 2 (n = 53) with no blastocyst. TaqMan human miRNA cards and quantitative reverse transcription polymerase chain reaction were used to identify differently expressed follicular fluid miRNAs between the two groups.Results: We found MIR-663B to be significantly differentially expressed in follicular fluid of oocytes that yielded viable blastocysts versus those that did not develop into blastocysts (14.16 +/- 7.00 versus 23.68 +/- 17.02; P = 0.019), as well as for those which develop into blastocysts with good morphology versus those with poor morphology (11.69 +/- 3.49 versus 20.16 +/- 9.33; P = 0.003).Conclusions: MIR-663B expression levels in human follicular fluid samples were significantly negatively related to viable blastocyst formation and may become an objective evaluation criterion for embryo development potential after IVF. (C) 2018 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.