Relationship between liver tissue stiffness and histopathological findings analyzed by shear wave elastography and compression testing in rats with non-alcoholic steatohepatitis
Relationship between liver tissue stiffness and histopathological findings analyzed by shear wave elastography and compression testing in rats with non-alcoholic steatohepatitis
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DOI：
10.1007/s10396-016-0698-9
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发表时间：
2016-07-01
影响因子：
1.8
通讯作者：
Sugimoto, Katsutoshi
中科院分区：
文献类型：
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作者：
Ogawa, Saori;Moriyasu, Fuminori;Sugimoto, Katsutoshi
本研究的目的是研究两种方法来测定由蛋氨酸和胆碱缺乏（MCD）饮食引起的不同程度的非酒精性脂肪性肝炎大鼠的肝脏硬度，并将每种结果与肝脏组织病理学结果进行比较。20只雄性Wister大鼠被喂食MCD饮食长达32周，另外4只喂食正常饮食。在指定时间点对每只大鼠进行基于超声的剪切波弹性成像（SWE）和Instron万能试验机的机械压缩测试。每次检查后，根据非酒精性脂肪性肝病（NAFLD）活动性评分分析肝脏组织病理学，以评估脂肪变性、炎症和纤维化的程度，并将每项发现与肝脏组织病理学结果进行比较。SWE测量的中位肝硬度值随着组织学炎症评分（P = 0.002）、肝纤维化分期（P = 0.029）、肿胀评分（P = 0.012）和脂肪变性等级（P = 0.030）的增加而逐步增加。使用Instron机测量的中位肝硬度显示，仅随着组织学纤维化分期的增加而逐步增加（P = 0.033）。SWE和Instron测量的肝脏僵硬程度不同。SWE主要反映炎症，而Instron机器值主要反映纤维化。这是用这两种方式进行的测量之间存在差异的主要原因。
The aim of the present study was to investigate two methods of determining liver stiffness in rats with various degrees of non-alcoholic steatohepatitis induced by a methionine- and choline-deficient (MCD) diet by comparing each finding with reference to histopathological liver findings.Twenty male Wister rats were fed an MCD diet for up to 32 weeks, and four were fed a normal diet. Ultrasound-based shear wave elastography (SWE) and mechanical compression testing using an Instron Universal Testing machine were performed on each rat at designated time points. After each examination, liver histopathology was analyzed to evaluate the degrees of steatosis, inflammation, and fibrosis based on non-alcoholic fatty liver disease (NAFLD) activity score, and each finding was compared with reference to liver histopathologic findings.Median liver stiffness values measured using SWE showed a stepwise increase with increasing histological inflammation score (P = 0.002), hepatic fibrosis stage (P = 0.029), ballooning score (P = 0.012), and steatosis grade (P = 0.030). Median liver stiffness measured using an Instron machine showed a stepwise increase only with increasing histological fibrosis stage (P = 0.033).Degree of liver stiffness measured by SWE and the Instron machine differed. SWE reflected mainly inflammation, whereas Instron machine-derived values primarily reflected fibrosis. This is the main source of discrepancies between measurements made with these two modalities.