Rapid spread of a symbiotic virus in a major crop pest following wide-scale adoption of Bt-cotton in China
Rapid spread of a symbiotic virus in a major crop pest following wide-scale adoption of Bt-cotton in China
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DOI：
10.1101/2021.02.08.430243
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发表时间：
2021-02
期刊：
影响因子：
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通讯作者：
Yutao Xiao;Wenjing Li;Xianming Yang;P. Xu;M. Jin;He Yuan;Weigang Zheng;M. Soberón;A. Bravo;K. Wilson;Kongming Wu
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文献类型：
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作者：
Yutao Xiao;Wenjing Li;Xianming Yang;P. Xu;M. Jin;He Yuan;Weigang Zheng;M. Soberón;A. Bravo;K. Wilson;Kongming Wu
苏云金芽孢杆菌（Bt）作物已被广泛种植，Bt作物对靶标害虫和非靶标害虫种群的影响已得到充分研究。然而，Bt作物暴露对与作物害虫相互作用的微生物的影响以前没有被量化。在这里，我们使用实验室和现场数据表明，感染棉铃虫与共生浓核病毒（HaDV 2）与其增强的生长和抗Bt棉。此外，田间监测表明，棉铃虫感染HaDV 2的发生率在种植Bt棉的地区比没有种植Bt棉的地区高得多，浓核病毒感染率随着Bt棉使用量的增加而增加。RNA-seq表明，通过免疫相关途径增强了对杆状病毒和Cry 1Ac的抗性。这表明，暴露于Bt作物选择了目标害虫和共生微生物之间的有益相互作用，增强了其在田间条件下对Bt作物的表现。
Bacillus thuringiensis (Bt) crops have been widely planted and the effects of Bt-crops on populations of the target and non-target insect pests were well studied. However, the effects of Bt-crops exposure on microorganisms that interact with crop pests haven’t previously been quantified. Here, we use laboratory and field data to show that infection of Helicoverpa armigera with a symbiotic densovirus (HaDV2) is associated with its enhanced growth and resistance to Bt-cotton. Moreover, field monitoring showed a much higher incidence of cotton bollworm infection with HaDV2 in regions cultivated with Bt-cotton than in regions without it, with the rate of densovirus infection increasing with increasing use of Bt-cotton. RNA-seq suggested resistance to both baculovirus and Cry1Ac were enhanced via the immune-related pathways. These suggest that the exposure to Bt-crops has selected for beneficial interactions between the target pest and a symbiotic microorganism that enhances its performance on Bt-crops under field conditions.