Disruption of pollen tube homogalacturonan synthesis relieves pollen tube penetration defects in the Arabidopsis O-FUCOSYLTRANSFERASE1 mutant
Disruption of pollen tube homogalacturonan synthesis relieves pollen tube penetration defects in the Arabidopsis O-FUCOSYLTRANSFERASE1 mutant
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DOI：
10.1007/s00497-023-00468-5
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发表时间：
2023-05
影响因子：
3.4
通讯作者：
Kayleigh Robichaux;Devin K. Smith;Madison Blea;Chrystle Weigand;J. Harper;I. Wallace
中科院分区：
文献类型：
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作者：
Kayleigh Robichaux;Devin K. Smith;Madison Blea;Chrystle Weigand;J. Harper;I. Wallace
在被子植物有性生殖过程中，花粉管必须穿过雌蕊中的多种细胞类型才能成功受精。虽然这个过程是高度编排的，需要复杂的化学和机械信号来引导花粉管到达目的地，但我们对花粉管穿透雌蕊的理解还不完整。我们以前的工作表明，拟南芥O-FUCOSYLTRANSFERASE 1（OFT 1）基因的破坏导致减少花粉管穿过柱头-花柱界面。在这里，我们证明，第二个位点突变的拟南芥半乳糖醛酸转移酶14（GAUT 14）有效地抑制表型oft 1突变体，部分恢复长角果长度，种子集，花粉传播，并在导航雌性生殖道的花粉管穿透缺陷。这些结果表明，果胶同型半乳糖醛酸（HG）合成的中断可以减轻与oft 1突变体相关的穿透缺陷，并可能暗示果胶HG沉积在拟南芥花粉管穿过柱头-花柱界面的过程中。这些结果也支持一个模型，其中OFT 1功能直接或间接修改与细胞壁相关的结构特征，与损失oft 1导致的壁组成的不平衡，可以通过减少果胶HG沉积补偿。
During angiosperm sexual reproduction, pollen tubes must penetrate through multiple cell types in the pistil to mediate successful fertilization. Although this process is highly choreographed and requires complex chemical and mechanical signaling to guide the pollen tube to its destination, aspects of our understanding of pollen tube penetration through the pistil are incomplete. Our previous work demonstrated that disruption of theArabidopsis thaliana O-FUCOSYLTRANSFERASE1 (OFT1)gene resulted in decreased pollen tube penetration through the stigma–style interface. Here, we demonstrate that second site mutations of ArabidopsisGALACTURONOSYLTRANSFERASE 14 (GAUT14)effectively suppress the phenotype ofoft1mutants, partially restoring silique length, seed set, pollen transmission, and pollen tube penetration deficiencies in navigating the female reproductive tract. These results suggest that disruption of pectic homogalacturonan (HG) synthesis can alleviate the penetrative defects associated with theoft1mutant and may implicate pectic HG deposition in the process of pollen tube penetration through the stigma–style interface in Arabidopsis. These results also support a model in which OFT1 function directly or indirectly modifies structural features associated with the cell wall, with the loss ofoft1leading to an imbalance in the wall composition that can be compensated for by a reduction in pectic HG deposition.