A rhodamine B-based lysosomal pH probe
A rhodamine B-based lysosomal pH probe
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DOI：
10.1039/c4tb01763c
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发表时间：
2015-01-01
影响因子：
7
通讯作者：
Zhao, Bao-Xiang
中科院分区：
文献类型：
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作者：
Shen, Shi-Li;Chen, Xin-Peng;Zhao, Bao-Xiang
通过将溶酶体靶向基团4-（2-氨乙基）吗啉部分整合到罗丹明B荧光团中，并与罗丹明B染料结合，开发了一种新的基于罗丹明B的溶酶体pH荧光探针（RML）。此外，RML具有良好的膜渗透性和各种氨基酸和金属阳离子之间的辉煌的选择性。在pH 7.40-4.00范围内，RML在583 nm处的荧光强度增加了80倍，pK（a）为5.16，表明RML在研究细胞内酸性细胞器方面具有重要价值。此外，RML已成功地应用于HeLa细胞，结果表明，RML可以选择性地染色活HeLa细胞中的溶酶体。注意，RML可用于检测HeLa细胞内由巴弗洛霉素A1诱导的溶酶体中的pH升高。
A novel rhodamine B-based fluorescent probe (RML) for lysosomal pH was developed by integrating a 4-(2-aminoethyl) morpholine moiety, which is a lysosome-targetable group, into a rhodamine B fluorophore, which is associated with rhodamine B dyes possessing spirocyclic (non-fluorescent) and ring-opening (fluorescent) forms with response to pH. The probe responded to acidic pH at low concentration in a short amount of time. In addition, RML showed good membrane permeability and brilliant selectivity among various amino acids and metal cations. RML exhibited an 80-fold increase in fluorescence intensity at 583 nm throughout the pH range of 7.40-4.00 with a pK(a) of 5.16, which indicates that RML is valuable for studying intracellular acidic organelles. Moreover, RML has been successfully applied in HeLa cells, and the results demonstrated that RML could selectively stain lysosomes in living HeLa cells. Note that RML could be used to detect the pH increase in lysosomes induced by bafilomycin A1 within HeLa cells.