Physicochemical changes and enzymatic activity of pre-harvest sprouting wheat
Physicochemical changes and enzymatic activity of pre-harvest sprouting wheat
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DOI：
10.5935/1806-6690.20150034
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发表时间：
2015-09-01
期刊：
影响因子：
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通讯作者：
Beleia, Adelaide Del Pino
中科院分区：
文献类型：
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作者：
Carvalho, Paulo de Tarso;Beleia, Adelaide Del Pino
在收获期间，小麦受到雨水的损害，这可能导致质量的变化和商业价值的损失。本研究的目的是描述巴西南部不同品种小麦的变化特征，这些小麦受到降雨的破坏，用下降数法检测。对13份样品进行百升重、千粒重、蛋白质、还原糖、可溶性氮、游离氨基氮和酶活性（α和β -淀粉酶、β -葡聚糖酶和蛋白酶）的评价。SDS-PAGE电泳检测麦胶蛋白组分的变化。还原糖、可溶性氮、游离氨基氮、α -淀粉酶、β -葡聚糖酶和蛋白酶活性均与下降数试验呈极显著相关。随着下降数值的减小，醇溶蛋白呈现出40和30 KDa波段的变化，且变化更加强烈。
During harvest, wheat is exposed to damage from rain, which may lead to changes in quality and loss of commercial value. The aim of this study was to characterize the changes in different varieties of wheat from the south of Brazil, damaged by rain as detected with the falling number method. Thirteen samples were evaluated for hectoliter weight, thousand-seed weight, protein, reducing sugars, soluble nitrogen, free amino nitrogen and enzyme activity (alpha and beta-amylase, beta-glucanase and protease). Changes in the gliadin fraction were evaluated by SDS-PAGE electrophoresis. The variables, reducing sugars, soluble nitrogen, free amino nitrogen, the activity of alpha-amylase, beta-glucanase and protease all showed significant correlation with the falling number test. As the falling number values were reduced, the gliadins displayed changes to the 40 and 30 KDa bands, which become more intense.