Transferrin 1 Functions in Iron Trafficking and Genetically Interacts with Ferritin in Drosophila melanogaster
Transferrin 1 Functions in Iron Trafficking and Genetically Interacts with Ferritin in Drosophila melanogaster
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转铁蛋白 1 在铁运输中发挥作用，并与果蝇中的铁蛋白发生遗传相互作用
DOI：
10.1016/j.celrep.2018.12.053
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发表时间：
2019
期刊：
影响因子：
8.8
通讯作者：
Zhou Bing
中科院分区：
文献类型：
--
作者：
Xiao Guiran;Liu Zhi-Hua;Zhao Mengran;Wang Hui-Li;Zhou Bing
铁代谢是一个必要的过程，当失调时会导致疾病。哺乳动物血清转铁蛋白(Tf)在向细胞运送铁的过程中起主要作用。为了更好地理解物种间铁代谢的保守性，我们在这里研究了果蝇转铁蛋白1(Tsf1)的转铁蛋白同源基因的功能。Tsf1基因敲除导致铁在肠道中积累和脂肪体(类似于哺乳动物肝脏)铁缺乏。脂肪体来源的Tsf1定位于肠道表面，表明Tsf1在肠道和脂肪体之间运输铁的功能类似于哺乳动物的Tf。此外，Tsf1基因敲除强烈抑制果蝇中已建立的铁贩子erritin(Fer1HCH)RNAi的表型效应。我们提出Tsf1和铁蛋白在果蝇中竞争铁，并证明了利用果蝇研究铁运输和全身铁调节的进化的价值。
Iron metabolism is an essential process that when dysregulated causes disease. Mammalian serum transferrin (TF) plays a primary role in delivering iron to cells. To improve our understanding of the conservation of iron metabolism between species, we investigate here the function of the TF homolog inDrosophila melanogaster, transferrin 1 (Tsf1).Tsf1knockdown results in iron accumulation in the gut and iron deficiency in the fat body (which is analogous to the mammalian liver). Fat body-derived Tsf1 localizes to the gut surface, suggesting that Tsf1 functions in trafficking iron between the gut and the fat body, similar to TF in mammals. Moreover,Tsf1knockdown strongly suppresses the phenotypic effects offerritin(Fer1HCH) RNAi, an established iron trafficker inDrosophila. We propose that Tsf1 and ferritin compete for iron in theDrosophilaintestine and demonstrate the value of usingDrosophilafor investigating iron trafficking and the evolution of systemic iron regulation.