Selenium Deficiency Deteriorate the Inflammation of S-aureus Infection via Regulating NF-κB and PPAR-γ in Mammary Gland of Mice
Selenium Deficiency Deteriorate the Inflammation of S-aureus Infection via Regulating NF-κB and PPAR-γ in Mammary Gland of Mice
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DOI：
10.1007/s12011-015-0563-5
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发表时间：
2016-07-01
影响因子：
3.9
通讯作者：
Zhang, Naisheng
中科院分区：
文献类型：
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作者：
Gao, Xuejiao;Zhang, Zecai;Zhang, Naisheng
硒（Se）是一种必需的微量营养素，有助于增强免疫系统，预防人类和动物的感染和疾病。硒具有调节机体免疫功能和抗炎作用。乳腺炎是一种由主要病原体S.金黄色。本研究旨在探讨硒对S.使用小鼠乳腺炎模型观察金黄色葡萄球菌诱导的炎症。免疫荧光染色检测组织病理学损伤。ELISA用于检测细胞因子表达，而蛋白质和mRNA水平分别通过Western印迹和qPCR分析进行分析。结果表明，硒缺乏增加了S。金黄色葡萄球菌感染的乳腺。低硒小鼠血清NO水平显著升高。金黄色乳腺炎硒缺乏促进促炎因子的产生，降低IL-10的表达。本研究结果还表明，硒对S.金黄色葡萄球菌诱导的乳腺炎与NF-κ B通路相关。事实上，硒缺乏抑制了PPAR-gamma活性，促进了NF-κ B通路的激活。因此，补硒可以改善对PPAR-gamma和NF-κ B的影响。提示硒缺乏可加重S.金黄色引起的乳腺炎。此外，本研究的结果有助于开发新的预防或治疗方法的S。金黄色葡萄球菌引起的乳腺炎和其他传染病。
Selenium (Se) is an essential micronutrient contributing to a strong immune system for the prevention of infections and diseases in humans and animals. Dietary Se regulates the immune status and mediates anti-inflammatory action. Mastitis is an inflammation in the mammary gland typically induced through the major pathogen S. aureus. The aim of the present study was to determine the regulating effect of Se on S. aureus-induced inflammation using a mouse mastitis model. Immunofluorescence staining was used to detect histopathological injury. ELISA was used to detect cytokine expression, while protein and mRNA levels were analyzed through Western blotting and qPCR analysis, respectively. The results showed that Se deficiency increased inflammatory lesions in individuals with S. aureus infection in the mammary gland. The NO levels showed a significant increase in Se-deficient mice with S. aureus mastitis. Se deficiency accelerated the production of pro-inflammatory factors and reduced IL-10 expression. Furthermore, the results of the present study showed that the regulating effect of Se on S. aureus-induced mastitis was associated with the NF-kappa B pathway. Indeed, Se deficiency suppressed PPAR-gamma activity and promoted NF-kappa B pathway activation. Thus, Se supplementation could improve the effect on PPAR-gamma and NF-kappa B. These results suggest that Se deficiency could aggravate the inflammatory injury resulting from S. aureus-induced mastitis. Moreover, the results of the present study contribute to the development of new prevention or treatment methods for S. aureus-induced mastitis and other infectious diseases.