Fucosylated glycoproteomic approach to identify a complement component 9 associated with squamous cell lung cancer (SQLC)
Fucosylated glycoproteomic approach to identify a complement component 9 associated with squamous cell lung cancer (SQLC)
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DOI：
10.1016/j.jprot.2011.07.019
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发表时间：
2011-11-18
影响因子：
3.3
通讯作者：
Cho, Je-Yoel
中科院分区：
文献类型：
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作者：
Narayanasamy, Arul;Ahn, Jung-Mo;Cho, Je-Yoel
人类肺癌是全世界癌症死亡率的主要原因。了解其病理生理学特征和开发用于诊断和治疗的新型生物标志物是主要任务。在本研究中，分别收集并合并来自10名SQLC患者和健康对照（HEC）的血清。通过免疫亲和方法耗尽合并的血清，并进一步进行岩藻糖基化富集。通过SDS-PAGE解析富集的岩藻糖基化糖蛋白，随后通过LC-ESI-MS/MS进行分析。从比较蛋白质组学分析中，我们选择了C9蛋白。采用Western blot、蛋白质芯片检测120例HEC和118例SQLC患者血清中C9蛋白水平，采用杂交凝集素ELISA（HLE）检测C9岩藻糖基化水平。通过Western印迹分析确定，SQLC患者的C9蛋白水平是HEC的6.4倍。结果同时得到了蛋白质阵列的证实，与HEC相比，SQLC患者的C9水平显著更高，灵敏度为53%，特异性为89%。SQLC患者的C9岩藻糖基化水平显著高于HEC（p
Human lung cancer is a major cause of cancer mortality worldwide. Understanding the pathophysiological features and the development of novel biomarkers for diagnosis as well as treatment are major tasks. In the present study, sera from ten SQLC patients and healthy control (HEC) were collected and pooled, respectively. The pooled sera were depleted via an immunoaffinity method and further subjected to fucosylation enrichment. Enriched fucosylated glycoproteins were resolved by SDS-PAGE and subsequently analyzed by LC-ESI-MS/MS. From comparative proteomic analysis, we selected the C9 protein. C9 protein levels were validated by Western blot, protein arrays and the fucosylation levels of C9 by hybrid lectin ELISA (HLE) in the sera of 120 HEC and 118 SQLC patients. The C9 protein level was 6.4-fold higher in SQLC patients compared to HEC, as determined by Western blot analysis. The results were concurrently confirmed by a protein array that showed a C9 level significantly higher in SQLC patients, as compared to HEC, with a sensitivity of 53% and a specificity of 89%. C9 fucosylation levels were significantly higher in SQLC patients compared to HEC (p