Comparative studies on manganese-based SOD mimetics, including the phosphate effect, by using global spectral analysis
Comparative studies on manganese-based SOD mimetics, including the phosphate effect, by using global spectral analysis
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DOI：
10.1016/j.jinorgbio.2011.12.008
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发表时间：
2012-04-01
影响因子：
3.9
通讯作者：
Ivanovic-Burmazovic, Ivana
中科院分区：
文献类型：
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作者：
Friedel, Felix C.;Lieb, Dominik;Ivanovic-Burmazovic, Ivana
停流测量已成功地与时间分辨紫外/可见光谱和超氧化物衰变的全局光谱分析相结合，用于文献中已知的假定的锰 SOD（超氧化物歧化酶）模拟物的催化活性的比较研究。所研究复合物的 SOD 活性按以下顺序降低：a) M40403 > Mn(III)TM-4-PyP5+ > Mn(II)pyane >> EUK-113，pH 7.4 (M40403 = ([二氯化锰(II){(4R,9R,14R,19R)-3,10,13,20,26-五氮四环[20.3.1.0.4,9014,19] hexacosa-1 (26),-22(23),24-三烯}], Mn(III)TM-4-PyP5+ = Mn(III)内消旋四(N-甲基吡啶鎓-4-基)卟啉，Mn(II)Pyane = Mn(II)trans-2.13-二甲基-3,6,9,12,18-五氮杂双环[12.3.1]-octadeca-1(18),14,16-thene，EUK-113 = [锰(III)乙酰基(6,6'-((1E,1'E)-(乙烷-1,2-二基双(氮杂亚基))双(亚甲基))双(2-甲氧基苯酚)])在Hepes缓冲液中(Hepes=4-(2-羟乙基)哌嗪-1-乙磺酸)，b) Mn(III)TM-4-PyP5+ > M40403与 Mn(II)pyane >> EUK-113（pH 8.1）一致，在 Hepes 缓冲液中； c) Mn(III)TM-4-PyP5+ 与 Mn(II)pyane 一致 > MnCl2> M40403 与 EUK-113（pH 7.4）一致，在磷酸盐缓冲液中。 Hepes 中的 MnCl2、Mn(III)TSPP3-、Mn(III)TBAP(3-)、Mn(III) 乙酸盐、Mn(II) 乳酸盐和 Mn(II)EDTA 不表现出 SOD 活性 (Mn(III)TSPP3- = Mn(III)mesotetrakis(苯甲酸)卟啉，Mn(III)TBAP(3-) = Mn(III)mesotetrakis(4-磺基苯基)卟啉)。 MnCl2 具有与磷酸盐存在下的其他模拟物相当的 SOD 活性。我们的结果表明，磷酸根阴离子独立于离子强度效应，影响锰 SOD 模拟物的活性，这对于理解和比较其生物活性至关重要。 (C) 2011 Elsevier Inc. 保留所有权利。
Stopped-flow measurements have been successfully combined with time-resolved UV/vis spectroscopy and global spectral analysis of superoxide decay for the comparative study of catalytic activity of putative manganese SOD (superoxide dismutase) mimetics known in the literature. The SOD activity of the studied complexes decreases in the following order: a) M40403 > Mn(III)TM-4-PyP5+ > Mn(II)pyane >> EUK-113 at pH 7.4 (M40403 = ([manganese(II)dichlorido{(4R,9R,14R,19R)-3,10,13,20,26-pentaazatetracyclo[20.3.1.0.4,9014,19] hexacosa-1 (26),-22( 23),24-triene}], Mn(III)TM-4-PyP5+ = Mn(III)meso-tetrakis(N-methylpyridnium-4-yl) porphyrin, Mn(II)Pyane = Mn(II)trans-2.13-dimethyl-3,6,9,12,18-pentaazabicyclo[12.3.1]-octadeca-1(18),14,16-thene, EUK-113 = [manganese(III)acetato(6,6'-((1E,1'E)-(ethane-1,2-diylbis(azanylylidene))bis(methanylylidene)) bis(2-methoxyphenolate)]) in Hepes buffer (Hepes=4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic add), b) Mn(III)TM-4-PyP5+ > M40403 congruent to Mn(II)pyane >> EUK-113 at pH 8.1 in Hepes buffer and c) Mn(III)TM-4-PyP5+ congruent to Mn(II)pyane > MnCl2> M40403 congruent to EUK-113 at pH 7.4 in phosphate buffer. MnCl2 in Hepes, Mn(III)TSPP3-, Mn(III)TBAP(3-), Mn(III)acetate, Mn(II)lactate and Mn(II)EDTA do not exhibit SOD activity (Mn(III)TSPP3- = Mn(III)mesotetrakis(benzoic acid)porphyrin, Mn(III)TBAP(3-) = Mn(III)mesotetrakis(4-sulfonatophenyl)porphyrin). MnCl2 possess SOD activity that is comparable with those of other mimetics in the presence of phosphate. Our results demonstrate that phosphate anions, independent of ionic strength effect, influence the activity of manganese SOD mimetics, which is crucial for understanding and comparing their biological activity. (C) 2011 Elsevier Inc. All rights reserved.