Simple multichannel system for the measurement of the net water flux across biological tissues.
Simple multichannel system for the measurement of the net water flux across biological tissues.
复制标题
用于测量跨生物组织的净水通量的简单多通道系统。
DOI：
10.1016/j.cmpb.2006.09.016
复制
发表时间：
2007
影响因子：
6.1
通讯作者：
Ibarra,Cristina
中科院分区：
文献类型：
--
作者：
Fernandez-Miyakawa,MarianoE;Dorr,Ricardo;Fernandez,LuisE;Uzal,FranciscoA;Ibarra,Cristina
本文介绍了一个简单的系统的发展，用于测量净水运动通过生物膜屏障。该系统是基于检测聚乙烯管内的水弯月面，它反映了一个hemichamber内的水运动的一个修改后的Ussing室含有膜屏障。检测装置由一台商用计算机控制的平板扫描仪和专门开发的软件组成。该系统允许每个物理单元执行相对高数量的单独实验。它是一种灵活和负担得起的设备，它允许获得比先前描述的方法相对更多的每单位信息。
This paper describes the development of a simple system for measurement of net water movement through biological membrane barriers. The system is based on the detection of a water meniscus inside a polyethylene tube, which reflects the water movement inside one hemichamber of a modified Ussing chamber containing a membrane barrier. The detection device consists of a commercial computer-controlled flat bed scanner and specifically developed software. This system allows one to perform a relatively high number of individual experiments per physical unit. It is a flexible and affordable device, which allows comparatively more information per unit to be obtained than previously described methods.