A conserved family of enzymes that phosphorylate inositol hexakisphosphate
A conserved family of enzymes that phosphorylate inositol hexakisphosphate
复制标题
DOI：
10.1126/science.1139099
复制
发表时间：
2007-04-06
期刊：
影响因子：
56.9
通讯作者：
York, John D.
中科院分区：
文献类型：
--
作者：
Mulugu, Sashidhar;Bai, Wenli;York, John D.
肌醇焦磷酸是一组不同的高能信号分子，其细胞作用仍然是一个活跃的研究领域。我们报告了一类以前未表征的肌醇焦磷酸合酶，并发现它与酵母 Vip1 和 Asp1 蛋白相同，即肌动蛋白相关蛋白 2/3 (ARP 2/3) 复合物的调节因子。 Vip1 和 Asp1 充当编码肌醇六磷酸 (IP6) 和肌醇七磷酸 (IP7) 激酶活性的酶。激酶活性的改变导致细胞生长、形态以及与 ARP 复合体成员相互作用的缺陷。 Asp1 和 Vip1 的功能可能通过 IP6 的代谢为细胞提供增强的信号传导能力。
Inositol pyrophosphates are a diverse group of high-energy signaling molecules whose cellular roles remain an active area of study. We report a previously uncharacterized class of inositol pyrophosphate synthase and find it is identical to yeast Vip1 and Asp1 proteins, regulators of actin-related protein-2/3 (ARP 2/3) complexes. Vip1 and Asp1 acted as enzymes that encode inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase activities. Alterations in kinase activity led to defects in cell growth, morphology, and interactions with ARP complex members. The functionality of Asp1 and Vip1 may provide cells with increased signaling capacity through metabolism of IP6.