Atmospheric Non-Equilibrium Plasma Promotes Cell Death and Cell-Cycle Arrest in a Lymphoma Cell Line
Atmospheric Non-Equilibrium Plasma Promotes Cell Death and Cell-Cycle Arrest in a Lymphoma Cell Line
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DOI：
10.1002/ppap.201500033
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发表时间：
2015-12-01
影响因子：
3.5
通讯作者：
Fimognari, Carmela
中科院分区：
文献类型：
--
作者：
Gherardi, Matteo;Turrini, Eleonora;Fimognari, Carmela
大气非平衡等离子体作为一种有前景的癌症治疗工具，由于其物理和化学成分的混合可以发挥抗肿瘤作用而引起了人们的兴趣。在这项工作中，我们研究了利用纳秒高压脉冲驱动的棒电极DBD进行的等离子体处理对小鼠淋巴瘤细胞的存活率、增殖和细胞周期分布的影响。比较了直接处理和间接处理的结果，前者将细胞悬浮在培养液中，而后者暴露在血浆中，后者将细胞加入先前经等离子体处理激活的培养液中。利用高速和ICCD成像对生物实验中不同操作条件下产生的等离子体放电进行了定性表征，并对培养基中等离子体处理产生的反应物种进行了半定量研究。
Atmospheric non-equilibrium plasma is drawing interest as a promising tool for cancer treatment due to its blend of physical and chemical components that can exert anti-tumor effects. In this work, we investigate the effects of plasma treatment, performed by means of a wand electrode DBD driven by nanosecond high voltage pulses, on the viability, proliferation, and cell-cycle distribution of cells of mouse lymphoma. Results for direct treatment, with cell exposed to plasma while suspended in culture medium, and indirect treatment, where cells were added to culture medium previously activated by plasma treatment, are compared. A qualitative characterization by means of high-speed and iCCD imaging of the plasma discharges produced for the different operating conditions adopted in biological experiments, as well as a semi-quantitative study of the reactive species produced by plasma treatment in the culture medium, are also presented.