70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria.
70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria.
复制标题
70 kD热休克相关蛋白是至少两个不同的胞质因子之一，刺激蛋白进口到线粒体中。
DOI：
10.1083/jcb.107.6.2051
复制
发表时间：
1988-12
影响因子：
7.8
通讯作者：
Blobel, G
中科院分区：
文献类型：
--
作者：
Murakami, H;Pain, D;Blobel, G
We have developed an in vitro system in which the posttranslational import of Put2 (delta-pyrroline-5-carboxylate dehydrogenase), into yeast mitochondria is dependent on the addition of yeast postribosomal supernatant (PRS). When mRNA for a nuclear-encoded yeast mitochondrial matrix protein, Put2, was translated in a wheat germ cell-free system, import into posttranslationally added yeast mitochondria was negligible. However, when a yeast PRS was added, significant import was observed. The import stimulating activity of the yeast PRS was shown to consist of at least two distinct factors. One of these is the recently purified 70-kD heat shock-related protein Ssalp/Ssa2p, two proteins that are 98% homologous. The other factor is an N-ethylmaleimide- sensitive protein(s). Both factors act synergistically.
